Tumor microenvironment profoundly modifies functional status of macrophages: peritoneal and tumor-associated macrophages are two very different subpopulations.
Tumor microenvironment profoundly modifies functional status of macrophages: peritoneal and tumor-associated macrophages are two very different subpopulations.
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DOI：
10.1016/j.cellimm.2013.06.008
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发表时间：
2013-05
影响因子：
4.3
通讯作者：
Torroella-Kouri M
中科院分区：
文献类型：
--
作者：
Rodríguez D;Silvera R;Carrio R;Nadji M;Caso R;Rodríguez G;Iragavarapu-Charyulu V;Torroella-Kouri M
巨噬细胞是炎症反应的关键参与者。在这项研究中，我们测试了这样一个假设，即尽管肿瘤宿主中的所有巨噬细胞亚群都受到疾病的影响，但正是与肿瘤的紧密接近引起了这些细胞的重大变化。我们比较了荷D1-DMBA-3乳腺肿瘤（T-PEM）小鼠的肿瘤相关巨噬细胞（TAM）和腹腔巨噬细胞。我们的研究结果表明，TAM下调IL-12 p70，但上调IL-12 p40，IL-23，IL-6和IL-10。TAM中一些NFκB和C/EBP转录因子家族成员减少;然而NFκ Bp 50同源二聚体、STAT 1/pSTAT 1和STAT 3/pSTAT 3过表达。此外，虽然TAM阻断T细胞增殖并且与T-PEM相比更易于凋亡，但两种类型的巨噬细胞都具有受损的吞噬能力。此外，TAM组成性表达iNOS，产生一氧化氮，但不表达NOS，Gr-1高，CD 11低。总的来说，我们对荷瘤小鼠中两个空间上不同的巨噬细胞亚群的分析表明，肿瘤将它们不同地调节成两个分子和功能上不同的巨噬细胞亚群。
Macrophages are key players in the inflammatory response. In this study, we tested the hypothesis that although all macrophage subpopulations in tumor hosts are affected by the disease, it is the close proximity to the tumor that induces major alterations in these cells. We compared tumor-associated macrophages (TAMs) with peritoneal macrophages from mice bearing D1-DMBA-3 mammary tumors (T-PEMs). Our results show that TAMs downregulate IL-12p70 but upregulate IL-12p40, IL-23, IL-6 and IL-10. Some NFκB and C/EBP transcription factors family members are decreased in TAMs; however NFκBp50 homodimers, STAT1/pSTAT1 and STAT3/pSTAT3 are overexpressed. Furthermore, while TAMs block T-cell proliferation and are more prone to apoptosis compared to T-PEMs, both types of macrophages have an impaired phagocytic capacity. Moreover, TAMs constitutively express iNOS and produce nitric oxide but do not express arginase and are Gr-1high and CD11blow. Collectively, our analysis of two spatially distinct macrophage subpopulations in tumor-bearing mice revealed that the tumor modulates them differently into two molecularly and functionally dissimilar macrophage subpopulations.
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核因子 - 卡帕布和肿瘤相关的巨噬细胞。
DOI：
10.1158/1078-0432.ccr-09-1015
发表时间：
2010-02-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Mancino A;Lawrence T
通讯作者：
Lawrence T
影响因子：
11.2
作者：
Movahedi, Kiavash;Laoui, Damya;Van Ginderachter, Jo A.
通讯作者：
Van Ginderachter, Jo A.
影响因子：
20.3
作者：
Biswas, SK;Gangi, L;Sica, A
通讯作者：
Sica, A
影响因子：
5.2
作者：
Caso, Raul;Silvera, Risset;Torroella-Kouri, Marta
通讯作者：
Torroella-Kouri, Marta
影响因子：
50.3
作者：
Kortylewski M;Xin H;Kujawski M;Lee H;Liu Y;Harris T;Drake C;Pardoll D;Yu H
通讯作者：
Yu H