Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens.
Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens.
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DOI：
10.1002/advs.202301643
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发表时间：
2023-08
期刊：
影响因子：
15.1
通讯作者：
Cooper, Jonathan M.
中科院分区：
文献类型：
--
作者：
Ibarra-Chavez, Rodrigo;Reboud, Julien;Penades, Jose R.;Cooper, Jonathan M.
Phage‐inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user‐friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL−1. In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell‐free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits. In this report, a new PICI‐based platform is showcased to detect and identify bacteria. In contrast to other diagnostic approaches, this strategy can additionally distinguish whether the pathogens are alive. PICI‐based infection of target hosts is simple and efficient, allowing us to demonstrate the implementation of the technique in an easy‐to‐use paper‐based format.
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