Pathway of nickel uptake influences its interaction with heterochromatic DNA.
Pathway of nickel uptake influences its interaction with heterochromatic DNA.
复制标题
镍摄取途径影响其与异染色质 DNA 的相互作用。
DOI：
10.1016/0041-008x(86)90135-3
复制
发表时间：
1986
影响因子：
3.8
通讯作者：
Max Costa
中科院分区：
文献类型：
--
作者：
Pramila Sen;Max Costa
将完整的中国仓鼠卵巢细胞暴露于水溶性 NiCl2 和颗粒结晶 NiS 中，会诱导浓度依赖性的染色体畸变发生率，包括间隙、断裂和交换。细胞暴露于结晶硫化镍颗粒会引起染色单体交换和双着丝粒的高发生率，并对 X 染色体异染色质长臂的凝聚状态产生影响。用NiCl2处理细胞不会对X染色体长臂造成任何显着影响，并且与NiS相比，双着丝粒型染色体畸变的发生率要低得多。为了检查结晶 NiS 颗粒产生的 X 染色体长臂的断裂/解缩是否是由于摄取 NiS 颗粒的吞噬途径所致，用封装在脂质体中的 NiCl2-白蛋白复合物处理细胞。虽然用 NiCl2-白蛋白复合物处理细胞产生的细胞内镍水平高于用 NiCl2 处理细胞所获得的细胞内镍水平，但在相当的细胞内水平下，当通过脂质体递送镍 (II) 时，观察到 X 染色体异色长臂的断裂/解缩，而当用未封装的 NiCl2 处理细胞时则观察不到。单独的离子镍，无论其传递机制如何，都表现出对异染色质的某种偏好，因为在染色体的异染色质着丝粒区域中观察到的畸变发生率较高。这些观察结果表明，从 NiS 颗粒传递 Ni2+ 的途径可能是该金属与异染色质优先相互作用的原因，从而影响 X 染色体异染色质长臂的凝聚态/断裂。
Exposure of intact Chinese hamster ovary cells to water-soluble NiCl2and to particulate crystalline NiS induced a concentration-dependent incidence of chromosomal aberrations which included gaps, breaks, and exhanges. Exposure of cells to crystalline NiS particles caused a high incidence of chromatid exchanges and dicentrics and produced what appears to be an effect on the condensation state of the heterochromatic long arm of the X chromosome. Treatment of cells with NiCl2did not cause any significant effect on the long arm of the X chromosome, and there was a much lower incidence of the dicentric type of chromosomal aberrations compared to NiS. To examine whether the fragmentation/decondensation of the long arm of the X chromosome produced by crystalline NiS particles was due to a phagoctyic pathyway of uptake of NiS particles, cells were treated with NiCl2-albumin complexes that had been encapsulated in liposomes. Although treatment of cells with NiCl2-albumin complexes yielded higher intracellular nickel levels than were obtained by treatment of cells with NiCl2, at comparable intracellular levels fragmentation/decondensation of the heterochromatic long arm of the X chromosome was observed when nickel (II) was delivered by way of a liposome but not when cells were treated with unencapsulated NiCl2. Ionic nickel alone irrespective of its delivery mechanism exhibited some preference for heterochromatin, since there was a higher incidence of aberrations observed in the heterochromatic centromeric region of chromosomes. These observations suggest that the pathway of delivery of Ni2+from NiS particles may be responsible for a preferential interaction of this metal with heterochromatin leading to an effect on the condensation state/fragmentation of the heterochromatic long arm of the X chromosome.
培养哺乳动物细胞中结晶硫化镍颗粒的吞噬作用和细胞内命运的视频延时显微镜。
影响因子：
11.2
作者：
Evans,RM;Davies,PJ;Costa,M
通讯作者：
Costa,M
影响因子：
11.2
作者：
J. Gilman
通讯作者：
J. Gilman