Recombinant human endostatin endostar inhibits tumor growth and metastasis in a mouse xenograft model of colon cancer.
Recombinant human endostatin endostar inhibits tumor growth and metastasis in a mouse xenograft model of colon cancer.
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DOI：
10.1007/s12253-011-9447-y
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发表时间：
2012-04
影响因子：
2.8
通讯作者：
Li, Zhongxin
中科院分区：
文献类型：
--
作者：
Jia, Yitao;Liu, Min;Huang, Wangang;Wang, Zhenbao;He, Yutong;Wu, Jianhua;Ren, Shuguang;Ju, Yingchao;Geng, Ruichao;Li, Zhongxin
研究重组人内皮抑素Endostar对小鼠大肠癌移植瘤转移、血管生成和淋巴管生成的影响。将结肠癌细胞SW 620接种于裸鼠左后腹皮下，建立裸鼠移植瘤模型。每隔一天用生理盐水或Endostar皮下处理小鼠。检测肿瘤细胞的生长、淋巴结转移、肿瘤组织中的血管生成和淋巴管生成。流式细胞仪检测细胞凋亡和细胞周期分布。通过免疫印迹分析测定SW 620细胞中VEGF-A、-C或-D的表达。恩度对肿瘤生长和淋巴结转移有明显抑制作用（P < 0.01）。恩度治疗组和对照组肿瘤内或周围血管密度分别为12.27 ± 1.21和22.25 ± 2.69个/视野（P < 0.05）。肿瘤组织中淋巴管密度明显降低（7.84 ± 0.81 vs.13.83 ± 1.08，P < 0.05）。恩度同时抑制转移淋巴结的血管生成和淋巴管生成。Endostar处理后SW 620细胞VEGF-A、-C和-D的表达明显低于对照组。恩度通过抑制小鼠结肠癌移植瘤模型中的血管生成和淋巴管生成来抑制结肠癌细胞的生长和淋巴结转移。
To investigate the effects of recombinant human endostatin Endostar on metastasis and angiogenesis and lymphangiogenesis of colorectal cancer cells in a mouse xenograft model. Colon cancer cells SW620 were injected subcutaneously into the left hind flank of nude mice to establish mouse xenograft models. The mice were treated with normal saline or Endostar subcutaneously every other day. The growth and lymph node metastasis of tumor cells, angiogenesis and lymphangiogenesis in tumor tissue were detected. Apoptosis and cell cycle distribution were studied by flow cytometry. The expression of VEGF-A, -C, or -D in SW620 cells was determined by immunoblotting assays. Endostar inhibited tumor growth and the rate of lymph node metastasis (P < 0.01). The density of blood vessels in or around the tumor area was 12.27 ± 1.21 and 22.25 ± 2.69 per field in Endostar-treated mice and controls (P < 0.05), respectively. Endostar also decreased the density of lymphatic vessels in tumor tissues (7.84 ± 0.81 vs. 13.83 ± 1.08, P < 0.05). Endostar suppresses angiogenesis and lymphangiogenesis in the lymph nodes with metastases, simultaneously. The expression of VEGF-A, -C and -D in SW620 cells treated with Endostar was substantially lower than that of controls. Endostar inhibited growth and lymph node metastasis of colon cancer cells by inhibiting angiogenesis and lymphangiogenesis in a mouse xenograft model of colon cancer.
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影响因子：
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