Gene expression in archived newborn blood spots distinguishes infants who will later develop cerebral palsy from matched controls.
Gene expression in archived newborn blood spots distinguishes infants who will later develop cerebral palsy from matched controls.
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DOI：
10.1038/pr.2012.200
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发表时间：
2013-04
影响因子：
3.6
通讯作者：
Paneth, Nigel
中科院分区：
文献类型：
--
作者：
Nhan Thi Ho;Furge, Kyle;Fu, Wenjiang;Busik, Julia;Khoo, Sok Kean;Lu, Qing;Lenski, Madeleine;Wirth, Julia;Hurvitz, Edward;Dodge, Nancy;Resau, James;Paneth, Nigel
在新生儿筛查中保留的存档新生儿血斑中的基因表达可能反映了有助于理解脑瘫（CP）病因的病理生理学紊乱。我们量化了代表四种生理途径的基因集的表达，这些生理途径假设有助于53名CP儿童和53名年龄、性别和孕龄匹配的对照组的存档未冷冻残留新生儿血斑标本中的CP。我们选择了四个经验和三个典型的基因集，代表炎症，缺氧，凝血和甲状腺途径，并使用8×60K寡核苷酸芯片检测mRNA表达。使用通用基因集富集（GAGE）方法分析匹配病例和对照之间基因表达的log2倍数变化。与匹配的对照组相比，足月出生的CP病例（N = 33）中的经验性炎症和经验性缺氧基因组显着下调（分别为P = 0.0007和0.0009），而这两个基因组显着上调（P = 0.0055和0.0223）早产儿CP病例（N = 20）。经验甲状腺基因组显着上调早产儿CP（P = 0.0023）。新生儿血斑转录组可以作为一个平台，用于研究后来发展为CP的儿童的独特基因表达模式。
Gene expression in archived newborn blood spots remaining from newborn screening may reflect pathophysiological disturbances useful in understanding the etiology of cerebral palsy (CP). We quantified the expression of gene sets representing four physiological pathways hypothesized to contribute to CP in archived unfrozen residual newborn blood spot specimens from 53 children with CP and 53 age, gender, and gestational-age–matched controls. We selected four empirical and three canonical gene sets representing inflammatory, hypoxic, coagulative, and thyroidal pathways, and examined mRNA expression using an 8×60K oligonucleotide microarray. The log2 fold change of gene expression between matched cases and controls were analyzed using the Generally Applicable Gene Set Enrichment (GAGE) method. The empirical inflammatory and empirical hypoxic gene sets were significantly down-regulated in term-born CP cases (N = 33) as compared to matched controls (P = 0.0007 and 0.0009, respectively), while both gene sets were significantly up-regulated (P = 0.0055 and 0.0223, respectively) in preterm-born CP cases (N = 20). The empirical thyroidal gene set was significantly up-regulated in preterm-born CP (P = 0.0023). The newborn blood spot transcriptome can serve as a platform for investigating distinctive gene expression patterns in children who later develop CP.
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DOI：
10.1111/j.1600-0897.2009.00791.x
发表时间：
2010-01
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
--
作者：
Madsen-Bouterse SA;Romero R;Tarca AL;Kusanovic JP;Espinoza J;Kim CJ;Kim JS;Edwin SS;Gomez R;Draghici S
通讯作者：
Draghici S
影响因子：
5.6
作者：
Resau JH;Ho NT;Dykema K;Faber MS;Busik JV;Nickolov RZ;Furge KA;Paneth N;Jewell S;Khoo SK
通讯作者：
Khoo SK
影响因子：
3
作者：
Luo W;Friedman MS;Shedden K;Hankenson KD;Woolf PJ
通讯作者：
Woolf PJ
影响因子：
5.8
作者：
Goeman, Jelle J.;Buehlmann, Peter
通讯作者：
Buehlmann, Peter
影响因子：
7
作者：
Clément, K;Viguerie, N;Langin, D
通讯作者：
Langin, D