The transcriptome of the fetal inflammatory response syndrome.
The transcriptome of the fetal inflammatory response syndrome.
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DOI：
10.1111/j.1600-0897.2009.00791.x
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发表时间：
2010-01
期刊：
影响因子：
--
通讯作者：
Draghici S
中科院分区：
文献类型：
--
作者：
Madsen-Bouterse SA;Romero R;Tarca AL;Kusanovic JP;Espinoza J;Kim CJ;Kim JS;Edwin SS;Gomez R;Draghici S
胎儿炎症反应综合征（FIRS）被认为是系统性炎症反应综合征（SIRS）的对应物，但它们的调节机制的相似性尚不清楚。本研究表征了胎儿外周血白细胞mRNA转录组，以确定FIRS中涉及的关键生物学过程和途径。新生儿出生时采集FIRS（宫颈炎，血浆IL-6 bb0 11 pg/ml， n=10）和无炎症迹象（n=10）的脐带血。白细胞RNA的微阵列分析显示541个独特基因的差异表达，44个基因中有41个通过qRT-PCR证实了变化。与SIRS和败血症类似，本体论和途径分析发现，抗原加工和递呈、免疫反应和细胞代谢关键过程等生物过程显著富集。结果与内毒素攻击模型和儿童败血症的微阵列研究相当，在所有研究中确定了25个基因。这项研究首次分析了FIRS的全基因组表达，尽管胎儿和成人免疫系统存在差异，但它与SIRS具有很大程度的相似性。
The fetal inflammatory response syndrome (FIRS) is considered the counterpart of the systemic inflammatory response syndrome (SIRS), but similarities in their regulatory mechanisms are unclear. This study characterizes the fetal mRNA transcriptome of peripheral leukocytes to identify key biological processes and pathways involved in FIRS. Umbilical cord blood from preterm neonates with FIRS (funisitis, plasma IL-6>11 pg/ml; n=10) and neonates with no evidence of inflammation (n=10) was collected at birth. Microarray analysis of leukocyte RNA revealed differential expression of 541 unique genes, changes confirmed by qRT-PCR for 41 or of 44 genes tested. Similar to SIRS and sepsis, ontological and pathway analyses yielded significant enrichment of biological processes including antigen processing and presentation, immune response, and processes critical to cellular metabolism. Results are comparable with microarray studies of endotoxin challenge models and pediatric sepsis, identifying 25 genes across all studies. This study is the first to profile genome-wide expression in FIRS, which demonstrates a substantial degree of similarity with SIRS despite differences in fetal and adult immune systems.
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影响因子：
3.6
作者：
Berner, R;Welter, P;Brandis, M
通讯作者：
Brandis, M
DOI：
10.1902/annals.2001.6.1.153
发表时间：
2001-12-01
期刊：
Annals of periodontology
影响因子：
--
作者：
Gibbs, R S
通讯作者：
Gibbs, R S
DOI：
10.1111/j.1600-0897.2007.00561.x
发表时间：
2008-01-01
影响因子：
3.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.4
作者：
Benjamim, CF;Canetti, C;Peters-Golden, M
通讯作者：
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