Oxygenation properties and oxidation rates of mouse hemoglobins that differ in reactive cysteine content.
Oxygenation properties and oxidation rates of mouse hemoglobins that differ in reactive cysteine content.
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DOI：
10.1016/j.cbpa.2011.11.004
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发表时间：
2012-02
影响因子：
2.3
通讯作者：
Fago, Angela
中科院分区：
文献类型：
--
作者：
Storz, Jay F.;Weber, Roy E.;Fago, Angela
House mice (genus Mus) harbor extensive allelic variation at two tandemly duplicated genes that encode the β-chain subunits of adult hemoglobin (Hb). Alternative haplotypes differ in the level of sequence divergence between the two β-globin gene duplicates: the Hbbdand Hbbphaplotypes harbor two structurally distinct β-globin genes, whereas the Hbbshaplotype harbors two β-globin duplicates that are identical in sequence. One especially salient difference between the s-type Hbs relative to the d- and p-type Hbs relates to the number of reactive β-chain cysteine residues. In addition to the highly conserved cysteine residue at β93, the d-and p-type Hbs contain an additional reactive cysteine residue at β13. To assess the functional consequences of allelic variation in β-globin cysteine content, we measured O2-binding properties and H2O2-induced oxidation rates of mono-and dicysteinyl β-Hbs from 4 different inbred strains of mice: C57BL/6J, BALB/cJ, MSM/Ms, and CAROLI/EiJ. The experiments revealed that purified Hbs from the various mouse strains did not exhibit substantial variation in O2-binding properties, but s-type Hb (which contains a single reactive β-chain cysteine residue) was far more readily oxidized to Fe3+ metHb by H2O2 than other mouse Hbs that contain two reactive β-chain cysteine residues. These results suggest that the possession of an additional reactive cysteine residue may protect against metHb formation under oxidizing conditions. The allelic differences in β-globin cysteine content could affect aspects of redox signaling and oxidative/nitrosative stress responses that are mediated by Hb-S-nitrosylation and Hb-S-glutathionylation pathways.
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DOI：
10.1085/jgp.43.4.737
发表时间：
1960-03-01
期刊：
The Journal of general physiology
影响因子：
--
作者：
Riggs A
通讯作者：
Riggs A
DOI：
10.1006/bbrc.2000.3326
发表时间：
2000-08-28
影响因子：
3.1
作者：
Miranda, JJ
通讯作者：
Miranda, JJ
影响因子：
3.9
作者：
Di Simplicio, P;Cacace, MG;Rossi, R
通讯作者：
Rossi, R
影响因子：
20.3
作者：
Peters, Luanne L.;Shavit, Jordan A.;Brugnara, Carlo
通讯作者：
Brugnara, Carlo
影响因子：
10.7
作者：
Hoffmann, Federico G.;Opazo, Juan C.;Storz, Jay F.
通讯作者：
Storz, Jay F.