Neuroprotective effects of testosterone on motoneuron and muscle morphology following spinal cord injury.
Neuroprotective effects of testosterone on motoneuron and muscle morphology following spinal cord injury.
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DOI：
10.1002/cne.23066
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发表时间：
2012-08-15
影响因子：
2.5
通讯作者：
Sengelaub, Dale R.
中科院分区：
文献类型：
--
作者：
Byers, James S.;Huguenard, Anna L.;Kuruppu, Dulanji;Liu, Nai-Kui;Xu, Xiao-Ming;Sengelaub, Dale R.
在各种运动神经元损伤后，睾酮治疗具有神经保护/神经治疗作用。在这里，我们评估了睾丸激素是否可能在脊髓损伤（SCI）后有类似的有益作用。年轻成年雌性大鼠接受假手术或T9脊髓挫伤，并植入空白或睾酮填充的硅橡胶胶囊。四周后，运动神经元支配股四头肌外侧肌标记霍乱毒素结合辣根过氧化物酶，和树突状乔木重建的三维。还评估了索马体积、运动神经元数量、病变体积和组织保留，以及肌肉重量、纤维横截面积和运动终板大小和密度。挫伤导致大的损伤，SCI组之间的损伤体积、损伤总体积百分比或保留的白色或灰质没有显著差异。SCI加或不加睾酮治疗对四头肌运动神经元的数量或索马体积也没有影响。然而，SCI导致未处理动物的四头肌运动神经元树突长度减少，而睾酮治疗完全阻止了这种减少。同样，未处理的SCI动物的股外侧肌肌肉重量和纤维横截面积小于假手术对照组，并且这些减少都被睾酮治疗所阻止。在各治疗组中未观察到对运动终板面积或密度的影响。这些研究结果表明，运动神经元和肌肉形态学的退行性变化，脊髓损伤后可以通过睾酮治疗，进一步支持睾酮作为神经治疗剂在损伤的神经系统中的作用。
Treatment with testosterone is neuroprotective/neurotherapeutic after a variety of motoneuron injuries. Here we assessed whether testosterone might have similar beneficial effects after spinal cord injury (SCI). Young adult female rats received either sham or T9 spinal cord contusion injuries and were implanted with blank or testosterone-filled Silastic capsules. Four weeks later, motoneurons innervating the vastus lateralis muscle of the quadriceps were labeled with cholera toxin-conjugated horseradish peroxidase, and dendritic arbors were reconstructed in three dimensions. Soma volume, motoneuron number, lesion volume, and tissue sparing were also assessed, as were muscle weight, fiber cross-sectional area, and motor endplate size and density. Contusion injury resulted in large lesions, with no significant differences in lesion volume, percent total volume of lesion, or spared white or gray matter between SCI groups. SCI with or without testosterone treatment also had no effect on the number or soma volume of quadriceps motoneurons. However, SCI resulted in a decrease in dendritic length of quadriceps motoneurons in untreated animals, and this decrease was completely prevented by treatment with testosterone. Similarly, the vastus lateralis muscle weights and fiber cross-sectional areas of untreated SCI animals were smaller than those of sham-surgery controls, and these reductions were both prevented by testosterone treatment. No effects on motor endplate area or density were observed across treatment groups. These findings suggest that regressive changes in motoneuron and muscle morphology seen after SCI can be prevented by testosterone treatment, further supporting a role for testosterone as a neurotherapeutic agent in the injured nervous system.
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影响因子：
5.1
作者：
BISBY, MA;TETZLAFF, W
通讯作者：
TETZLAFF, W
影响因子：
5.3
作者：
CastroMoure, F;Goshgarian, HG
通讯作者：
Goshgarian, HG
影响因子：
4.2
作者：
Fargo, Keith N.;Alexander, Thomas D.;Jones, Kathryn J.
通讯作者：
Jones, Kathryn J.
影响因子：
5.3
作者：
BERNSTEIN, JJ;STANDLER, NA
通讯作者：
STANDLER, NA
影响因子：
3
作者：
Fargo, Keith N.;Sengelaub, Dale R.
通讯作者：
Sengelaub, Dale R.