Two-photon microscopy using fiber-based nanosecond excitation.
Two-photon microscopy using fiber-based nanosecond excitation.
复制标题
使用基于光纤的纳秒激发的双光子显微镜。
作者：
S. Karpf;Matthias Eibl;B. Sauer;F. Reinholz;G. Hüttmann;R. Huber
双光子激发荧光（TPEF）显微镜是一种功能强大的技术，用于在高达1000微米的深度进行敏感组织成像。然而，由于穿透较浅，对于患者体内内脏器官的体内成像，通过内窥镜进行射束输送是至关重要的。直到今天，这还受到内窥镜光纤中飞秒脉冲的线性和非线性脉冲展宽的阻碍。在这里，我们提出了一个内窥镜准备，基于光纤的TPEF显微镜，使用纳秒脉冲在低重复率，而不是飞秒脉冲。这些纳秒脉冲没有与飞秒脉冲相关的大部分问题，但同样适用于TPEF成像。我们推导并证明，在给定的CW功率的TPEF信号只取决于占空比的激光源。由于在相同的峰值功率下具有更高的脉冲能量，我们还可以演示单次双光子荧光寿命测量。
Two-photon excitation fluorescence (TPEF) microscopy is a powerful technique for sensitive tissue imaging at depths of up to 1000 micrometers. However, due to the shallow penetration, for in vivo imaging of internal organs in patients beam delivery by an endoscope is crucial. Until today, this is hindered by linear and non-linear pulse broadening of the femtosecond pulses in the optical fibers of the endoscopes. Here we present an endoscope-ready, fiber-based TPEF microscope, using nanosecond pulses at low repetition rates instead of femtosecond pulses. These nanosecond pulses lack most of the problems connected with femtosecond pulses but are equally suited for TPEF imaging. We derive and demonstrate that at given cw-power the TPEF signal only depends on the duty cycle of the laser source. Due to the higher pulse energy at the same peak power we can also demonstrate single shot two-photon fluorescence lifetime measurements.
影响因子：
16.6
作者：
Karpf, Sebastian;Eibl, Matthias;Wieser, Wolfgang;Klein, Thomas;Huber, Robert
通讯作者：
Huber, Robert
影响因子：
56.9
作者：
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者：
WEBB, WW