A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy.
A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy.
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DOI：
10.1038/ncomms7784
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发表时间：
2015-04-17
影响因子：
16.6
通讯作者：
Huber, Robert
中科院分区：
文献类型：
--
作者：
Karpf, Sebastian;Eibl, Matthias;Wieser, Wolfgang;Klein, Thomas;Huber, Robert
拉曼传感和显微镜是最具体的光学技术，以确定未知样品的化学化合物，并使无标记的生物医学成像。在这里，我们提出了一种受激拉曼散射光谱和成像的时间编码（TICO）的拉曼概念的方法。我们使用连续波，快速波长扫描的探测激光器和联合收割机结合短占空比主动调制泵浦激光器。因此，我们实现了高受激拉曼增益信号电平，同时仍然受益于连续波操作的窄线宽和低噪声。我们的全光纤TICO拉曼装置使用傅立叶域锁模激光源，实现了高速、宽光谱覆盖范围（750- 3，150 cm−1）和高分辨率（0.5 cm−1）的独特组合。拉曼信息被直接编码并及时获取。我们展示了定量化学分析的溶剂混合物和高光谱拉曼显微镜与植物细胞的分子对比。 拉曼显微镜受到速度和光谱信息之间的折衷的影响，并且通常不适合光纤光束传输。Karpf等人在全光纤装置中使用连续波快速波长扫描探测激光器和短占空比主动调制泵浦激光器克服了这些限制。
Raman sensing and microscopy are among the most specific optical technologies to identify the chemical compounds of unknown samples, and to enable label-free biomedical imaging. Here we present a method for stimulated Raman scattering spectroscopy and imaging with a time-encoded (TICO) Raman concept. We use continuous wave, rapidly wavelength-swept probe lasers and combine them with a short-duty-cycle actively modulated pump laser. Hence, we achieve high stimulated Raman gain signal levels, while still benefitting from the narrow linewidth and low noise of continuous wave operation. Our all-fibre TICO-Raman setup uses a Fourier domain mode-locked laser source to achieve a unique combination of high speed, broad spectral coverage (750–3,150 cm−1) and high resolution (0.5 cm−1). The Raman information is directly encoded and acquired in time. We demonstrate quantitative chemical analysis of a solvent mixture and hyperspectral Raman microscopy with molecular contrast of plant cells. Raman microscopes suffer from the compromise between speed and spectral information and are often unsuited for fibre beam delivery. Karpf et al. overcome these limitations using continuous-wave rapidly wavelength-swept probe lasers and a short-duty-cycle actively modulated pump laser in an all-fibre setup.
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