Study on Caenorhabditis Elegans as a Combined Model of Microdosimetry and Biology.
Study on Caenorhabditis Elegans as a Combined Model of Microdosimetry and Biology.
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秀丽隐杆线虫作为微剂量学和生物学组合模型的研究
DOI：
10.1177/1559325821990125
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Chen N
中科院分区：
文献类型：
--
作者：
Yu W;Long H;Gao J;Wang Y;Tu Y;Sun L;Chen N
微剂量学是研究电离辐射微观能量沉积的一种工具。这项工作使用秀丽隐杆线虫作为模型，以估计微剂量沉积水平在60 Co γ辐射。采用蒙特卡罗软件PHITS建立辐射线虫模型。计算100戈伊照射后全身及性腺的剂量沉积。通过生物学指标的检测评价辐射损伤水平。微剂量学实验结果表明，线虫全身受照剂量为99.9 ± 57.8戈伊，范围为19.6 ~ 332.2戈伊。性腺受照剂量为129.4 ± 558.8戈伊（9.5 ~ 6597戈伊）。生物学实验结果表明，辐照后线虫的长度变化不大。但每分钟甩头次数和连续3天产卵量分别下降了27.1%和94.7%。辐照组的线虫表现出异质性。等高线分析表明，线虫的行为运动学和生殖能力变化趋势与微剂量学模型估算的剂量分布水平一致。最后，C. elegans提出了一个适合于研究辐射的微剂量学和生物学的综合模型。
Microdosimetry is a tool for the investigation of microscopic energy deposition of ionizing radiation. This work used Caenorhabditis elegans as a model to estimate the microdosimetric deposition level at the 60Co gamma radiation. Monte Carlo software PHITS was employed to establish irradiated nematodes model. The dose deposition of the entire body and gonad irradiated to 100 Gy was calculated. The injury levels of radiation were evaluated by the detection of biological indicators. The result of microdosimetric experiment suggested that the dose of whole body of nematodes was estimated to be 99.9 ± 57.8 Gy, ranging from 19.6 to 332.2 Gy. The dose of gonad was predicted to be 129.4 ± 558.8 Gy (9.5-6597 Gy). The result of biological experiment suggested that there were little changes in the length of nematodes after irradiation. However, times of head thrash per minute and the spawning yield in 3 consecutive days decreased 27.1% and 94.7%, respectively. Nematodes in the irradiated group displayed heterogeneity. Through contour analysis, trends of behavior kinematics and reproductive capacity of irradiated nematodes proved to be consistent with the dose distribution levels estimated by microdosimetric model. Finally, C. elegans presented a suitable combined model of microdosimetry and biology for studying radiation.
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影响因子：
2
作者：
Suzuki M;Hattori Y;Sakashita T;Yokota Y;Kobayashi Y;Funayama T
通讯作者：
Funayama T
影响因子：
64.8
作者：
Peng Y;Zhang M;Zheng L;Liang Q;Li H;Chen JT;Guo H;Yoshina S;Chen YZ;Zhao X;Wu X;Liu B;Mitani S;Yu JS;Xue D
通讯作者：
Xue D
线虫中辐射诱导的从体细胞到生殖细胞的旁观者信号传导
影响因子：
3.4
作者：
Guo, Xiaoying;Sun, Jie;Wu, Lijun
通讯作者：
Wu, Lijun
影响因子：
1.2
作者：
Nose, H;Niita, K;Kanai, T
通讯作者：
Kanai, T
DOI：
10.1126/science.1158111
发表时间：
2008-10-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Deng X;Yin X;Allan R;Lu DD;Maurer CW;Haimovitz-Friedman A;Fuks Z;Shaham S;Kolesnick R
通讯作者：
Kolesnick R