Multiple POT1-TPP1 proteins coat and compact long telomeric single-stranded DNA.
Multiple POT1-TPP1 proteins coat and compact long telomeric single-stranded DNA.
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DOI：
10.1016/j.jmb.2011.04.049
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发表时间：
2011-07-01
影响因子：
5.6
通讯作者：
Cech TR
中科院分区：
文献类型：
--
作者：
Taylor DJ;Podell ER;Taatjes DJ;Cech TR
Telomeres are nucleoprotein complexes that cap and protect the ends of linear chromosomes. In humans, telomeres end in 50 – 300 nucleotides of G-rich single-stranded DNA (ssDNA) overhangs. Protection of telomeres 1 (POT1) binds with nanomolar affinity to the ssDNA overhangs and forms a dimer with another telomere-end binding protein called TPP1. Whereas most previous studies utilized telomeric oligonucleotides comprising single POT1-TPP1 binding sites, here we examined 72 – 144 nucleotide tracts of telomeric DNA containing 6 – 12 POT1-TPP1 binding sites. Using electrophoretic mobility gel shift assays, size-exclusion chromatography, and electron microscopy, we analyzed telomeric nucleoprotein complexes containing POT1 alone, POT1-TPP1, and a truncated version of POT1 (POT1-N) that maintains its DNA-binding domain. The results revealed that POT1-N and POT1-TPP1 can completely coat long telomeric ssDNA substrates. Furthermore, we show that ssDNA coated with human POT1-TPP1 heterodimers forms compact, potentially ordered structures.
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DOI：
10.1126/science.1170633
发表时间：
2009-11-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
de Lange T
通讯作者：
de Lange T
影响因子：
21.3
作者：
Liu, D;Safari, A;Zhou, SY
通讯作者：
Zhou, SY
影响因子：
30.8
作者：
Broccoli, D;Smogorzewska, A;deLange, T
通讯作者：
deLange, T
影响因子：
16.8
作者：
Amiard, Simon;Doudeau, Michel;Giraud-Panis, Marie-Josephe
通讯作者：
Giraud-Panis, Marie-Josephe
影响因子：
30.8
作者：
Kim, SH;Kaminker, P;Campisi, J
通讯作者：
Campisi, J