Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates.
Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates.
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DOI：
10.1093/nar/gkad848
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发表时间：
2023-11-27
影响因子：
14.9
通讯作者：
中科院分区：
文献类型：
--
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Rdh 54是一种保守的DNA转位酶，参与同源重组（HR），DNA检查点适应和染色体分离。酿酒酵母Rdh 54是Mec 1/Rad 53信号传导轴的已知靶标，其在DNA代谢期间全面保护基因组完整性。虽然Mec 1/Rad 53对DNA修复蛋白的磷酸化对HR进展至关重要，但对特定的翻译后修饰如何改变HR反应知之甚少。Rdh 54的磷酸化与保护基因组完整性有关，但修饰的后果仍然知之甚少。在这里，我们证明了由效应激酶Rad 53调节Rdh 54的C-末端的磷酸化Rdh 54聚类活性所揭示的单分子成像。这源于Rdh 54和Rad 53之间的磷酸化依赖性和独立性相互作用。遗传分析表明，磷酸化的丢失导致表型变化，导致杂合性丢失（洛）的结果。我们的数据突出了Rad 53作为HR中间体的关键调节剂，通过激活和减弱Rdh 54运动功能。
Rdh54 is a conserved DNA translocase that participates in homologous recombination (HR), DNA checkpoint adaptation, and chromosome segregation. Saccharomyces cerevisiae Rdh54 is a known target of the Mec1/Rad53 signaling axis, which globally protects genome integrity during DNA metabolism. While phosphorylation of DNA repair proteins by Mec1/Rad53 is critical for HR progression little is known about how specific post translational modifications alter HR reactions. Phosphorylation of Rdh54 is linked to protection of genomic integrity but the consequences of modification remain poorly understood. Here, we demonstrate that phosphorylation of the Rdh54 C-terminus by the effector kinase Rad53 regulates Rdh54 clustering activity as revealed by single molecule imaging. This stems from phosphorylation dependent and independent interactions between Rdh54 and Rad53. Genetic assays reveal that loss of phosphorylation leads to phenotypic changes resulting in loss-of-heterozygosity (LOH) outcomes. Our data highlight Rad53 as a key regulator of HR intermediates through activation and attenuation of Rdh54 motor function.
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