Myosin heavy chain mRNA and protein expression in single fibers of the rat soleus following reinnervation
Myosin heavy chain mRNA and protein expression in single fibers of the rat soleus following reinnervation
复制标题
神经支配后大鼠比目鱼肌单纤维肌球蛋白重链 mRNA 和蛋白表达
DOI：
10.1016/s0304-3940(96)12926-8
复制
发表时间：
1996
影响因子：
2.5
通讯作者：
D. Pierotti
中科院分区：
文献类型：
--
作者：
S. Bodine;D. Pierotti
肌球蛋白重链（Myosin heavy chain， MHC）的表达受包括神经输入在内的多种因素调控。为了更好地理解再神经支配后肌球蛋白的转化，我们检测了比目鱼单根纤维中的MHC蛋白和mRNA。大腿中部坐骨神经病变导致比目鱼肌受到来自原始和外来运动池的运动神经元的神经支配。用原位组织化学和免疫组织化学方法检测损伤后8周和16周单个纤维中MHC的表达。在坐骨神经损伤后，经神经支配的比目鱼肌经历了由慢向快的生理生化变化。在8周时，快速MHC mRNA亚型（IIa和IIx）上调，慢速mRNA下调，然而，主要的蛋白质亚型是MHC i。在8周和16周时，许多纤维表达多种mRNA亚型。16周时，缓慢和快速MHC mrna的共表达有限，但快速MHC mrna继续共表达。再神经移植16周后，比目鱼中主要的快mRNA和蛋白为IIx MHC。
Myosin heavy chain (MHC) expression is regulated by many factors including neural input. To gain a better understanding of myosin transformation following reinnervation we examined both MHC protein and mRNA in single fibers of the soleus. A midthigh sciatic nerve lesion resulted in reinnervation of the soleus by motoneurons from both the original and foreign motor pools. MHC expression was examined in individual fibers 8 and 16 weeks post injury using in situ histochemistry and immunohistochemistry. Following a sciatic nerve lesion, the reinnervated soleus underwent a transformation from slow toward fast based on physiologic and biochemical measurements. At 8 weeks, fast MHC mRNA isoforms (IIa and IIx) were upregulated and slow mRNA was downregulated, however, the predominant protein isoform was MHC I. At both 8 and 16 weeks, many fibers expressed multiple mRNA isoforms. At 16 weeks there was limited co-expression of slow and fast MHC mRNAs, but continued co-expression of fast MHC mRNAs. Sixteen weeks following reinnervation the predominant fast mRNA and protein in the soleus was IIx MHC.
登录
查看更多内容
2A 型和 2B 型大鼠骨骼肌肌球蛋白尾区的克隆和原位杂交：对丝组装的影响。
影响因子：
3.1
作者：
Lieber,RL;Bodine,SC;Burkholder,TJ;Pierotti,DJ;Ryan,AF
通讯作者：
Ryan,AF
啮齿动物后肢骨骼肌中肌球蛋白同工酶的分布。
DOI：
10.1152/jappl.1986.60.6.1923
发表时间：
1986
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Thomason,DB;Baldwin,KM;Herrick,RE
通讯作者：
Herrick,RE
脊髓横断大鼠的比目鱼肌中肌球蛋白重链混合纤维的突出。
DOI：
10.1152/jappl.1995.78.4.1256
发表时间：
1995
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Talmadge,RJ;Roy,RR;Edgerton,VR
通讯作者：
Edgerton,VR
长期卸载肌肉中肌球蛋白重链谱的适应。
DOI：
--
发表时间：
1995
期刊：
Basic and applied myology : BAM
影响因子：
--
作者：
Talmadge,RJ;Roy,RR;Bodine-Fowler,SC;Pierotti,DJ;Edgerton,VR
通讯作者：
Edgerton,VR