Detection of low-level tumor cells in allergic contact dermatitis induced by mechlorethamine in patients with mycosis fungoides.
Detection of low-level tumor cells in allergic contact dermatitis induced by mechlorethamine in patients with mycosis fungoides.
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蕈样肉芽肿患者氮芥致过敏性接触性皮炎低水平肿瘤细胞的检测
DOI：
10.1111/1523-1747.ep12345517
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Wood,GS
中科院分区：
文献类型：
--
作者：
Veelken,H;Sklar,JL;Wood,GS
Two patients with histologically proven mycosis fungoides, a malignancy of phenotypically mature T cells, received a topical challenge with mechlorethamine to areas of clinically uninvolved skin to exclude possible hypersensitivity reactions to this chemotherapeutic agent. In both patients, allergic contact dermatitis (ACD) developed at the sites of the application and resolved completely after withdrawal of mechlorethamine. The lesions were biopsied and analyzed for the presence of clonal T-cell receptor (TCR)-γgene rearrangements using two polymerase chain reaction (PCR)-based assays involving denaturing gradient gel electrophoresis (PCR/DGGE) and ribonuclease protection analysis (PCR/RPA). The former method has a clonal detection threshold of 10−3−10−2, while the latter has a sensitivity of 10−5. In both cases, the ACD lesions were polyclonal by PCR/DGGE. In contrast, PCR/RPA detected tumor-specific TCR-γgene rearrangements in these same lesions. This indicates that the ACD lesions contained tumor cells at a density within the 10−5−102range. Analysis of peripheral blood mononuclear cells from both patients failed to detect the malignant done and showed the same result as blood from four normal individuals. The normal skin from one patient also lacked detectable TCR-γgene rearrangements. These results Indicate that mycosis fungoides tumor cells are present within ACD lesions Induced In mycosis fungoides patients and that this phenomenon does not appear to be due to the ubiquitous presence of detectable levels of these tumor cells in the blood or skin. These findings might be explained by nonspecific recruitment of malignant T cells to sites of local inflammation mediated by non-neoplastic antigen-specific T cells. Alternatively, they might be due to the local proliferation of very rare tumor cells in apparently normal skin in response to cytokines generated during the ACD reaction. In either case, the present study offers evidence that the malignant cells in mycosis fungoides retain at least some capability of respondingin vivoto physiologic stimuli.
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局部二甲氯乙胺化疗
影响因子：
3.6
作者：
E. Vonderheid
通讯作者：
E. Vonderheid
DOI：
10.1016/s0190-9622(89)70051-7
发表时间：
1989-03-01
影响因子：
13.8
作者：
VONDERHEID, EC;TAN, ET;VANSCOTT, EJ
通讯作者：
VANSCOTT, EJ
通过聚合酶链反应和变性梯度凝胶电泳 (PCR/DGGE) 检测早期蕈样肉芽肿/Sezary 综合征中克隆 T 细胞受体 γ 基因重排。
DOI：
10.1111/1523-1747.ep12389114
发表时间：
1994
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Wood,GS;Tung,RM;Haeffner,AC;Crooks,CF;Liao,S;Orozco,R;Veelken,H;Kadin,ME;Koh,H;Heald,P
通讯作者：
Heald,P
早期皮肤 T 细胞淋巴瘤的克隆性疾病。
影响因子：
2.4
作者：
V. Mielke;G. Staib;W. Boehncke;Bernd Duller;Wolfram Sterry
通讯作者：
Wolfram Sterry
影响因子：
10.3
作者：
PRICE, NM;CONSTANTINE, VS;FARBER, EM
通讯作者：
FARBER, EM