Peptidoglycan from Immunobiotic Lactobacillus rhamnosus Improves Resistance of Infant Mice to Respiratory Syncytial Viral Infection and Secondary Pneumococcal Pneumonia.
Peptidoglycan from Immunobiotic Lactobacillus rhamnosus Improves Resistance of Infant Mice to Respiratory Syncytial Viral Infection and Secondary Pneumococcal Pneumonia.
复制标题
DOI：
10.3389/fimmu.2017.00948
复制
发表时间：
2017
影响因子：
7.3
通讯作者：
Villena J
中科院分区：
文献类型：
--
作者：
Clua P;Kanmani P;Zelaya H;Tada A;Kober AKMH;Salva S;Alvarez S;Kitazawa H;Villena J
一些研究工作已经证明，具有调节粘膜免疫系统（免疫生物制剂）能力的有益微生物是改善细菌和病毒呼吸道感染结果的有趣替代方案。在具有有益调节呼吸免疫能力的免疫生物素菌株中，鼠李糖乳杆菌CRL 1505具有突出的特性。尽管我们在证明L.尽管在本发明的实施例中，已经发现了鼠李糖乳杆菌CRL 1505菌株用于改善对呼吸道感染的抗性以及在涉及其有益活性的细胞和分子机制中的作用，但是之前尚未解决该菌株或其免疫调节细胞级分在继发性细菌性肺炎的情况下的潜在保护能力。在这项工作中，我们证明了鼻预充与非活的L。鼠李糖乳杆菌CRL 1505或其纯化的肽聚糖差异性地调节由Toll样受体3激活在幼年小鼠中触发的呼吸道先天性抗病毒免疫应答，改善对原发性呼吸道合胞病毒（RSV）感染和继发性肺炎球菌肺炎的抗性。与抗RSV-肺炎球菌重叠感染相关，我们发现来自L。rhamnosus CRL 1505显著改善肺CD 3 + CD 4 +IFN-γ+和CD 3 + CD 4 +IL-10+ T细胞以及CD 11 c +SiglecF+IFN-β+肺泡巨噬细胞，从而增加呼吸道中的IFN-γ、IL-10和IFN-β。我们的研究结果还表明，这三种细胞因子的增加是必要的，以实现对呼吸道二重感染的保护，因为它们中的每一个都参与了继发性肺炎球菌肺炎的不同方面，必须加以控制，以减少感染性疾病的严重程度：肺肺炎球菌定植，菌血症，和炎症介导的肺组织损伤。
Several research works have demonstrated that beneficial microbes with the capacity to modulate the mucosal immune system (immunobiotics) are an interesting alternative to improve the outcome of bacterial and viral respiratory infections. Among the immunobiotic strains with the capacity to beneficially modulate respiratory immunity, Lactobacillus rhamnosus CRL1505 has outstanding properties. Although we have significantly advanced in demonstrating the capacity of L. rhamnosus CRL1505 to improve resistance against respiratory infections as well as in the cellular and molecular mechanisms involved in its beneficial activities, the potential protective ability of this strain or its immunomodulatory cellular fractions in the context of a secondary bacterial pneumonia has not been addressed before. In this work, we demonstrated that the nasal priming with non-viable L. rhamnosus CRL1505 or its purified peptidoglycan differentially modulated the respiratory innate antiviral immune response triggered by toll-like receptor 3 activation in infant mice, improving the resistance to primary respiratory syncytial virus (RSV) infection, and secondary pneumococcal pneumonia. In association with the protection against RSV-pneumococcal superinfection, we found that peptidoglycan from L. rhamnosus CRL1505 significantly improved lung CD3+CD4+IFN-γ+, and CD3+CD4+IL-10+ T cells as well as CD11c+SiglecF+IFN-β+ alveolar macrophages with the consequent increases of IFN-γ, IL-10, and IFN-β in the respiratory tract. Our results also showed that the increase of these three cytokines is necessary to achieve protection against respiratory superinfection since each of them are involved in different aspect of the secondary pneumococcal pneumonia that have to be controlled in order to reduce the severity of the infectious disease: lung pneumococcal colonization, bacteremia, and inflammatory-mediated lung tissue injury.
登录
查看更多内容
影响因子：
5.4
作者：
Avadhanula, V;Rodriguez, CA;Adderson, EE
通讯作者：
Adderson, EE
影响因子：
3.6
作者：
Hament, JM;Aerts, PC;Wolfs, TFW
通讯作者：
Wolfs, TFW
影响因子：
4.4
作者：
Mancuso, Giuseppe;Midiri, Angelina;Teti, Giuseppe
通讯作者：
Teti, Giuseppe
影响因子：
6.4
作者：
Parker D;Martin FJ;Soong G;Harfenist BS;Aguilar JL;Ratner AJ;Fitzgerald KA;Schindler C;Prince A
通讯作者：
Prince A
影响因子：
4.4
作者：
van der Sluijs, KF;van Elden, LJR;van der Poll, T
通讯作者：
van der Poll, T