Xyloglucan octasaccharide XXLGol derived from the seeds of hymenaea courbaril acts as a signaling molecule
Xyloglucan octasaccharide XXLGol derived from the seeds of hymenaea courbaril acts as a signaling molecule
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源自 hymenaea cobaril 种子的木葡聚糖八糖 XXLGol 作为信号分子
作者：
Vargas;Reicher;Rita Sierakowski M;Heyraud;Driguez;Linart
用Humicola indo1,4- β -d-葡聚糖酶I处理土膜草种子中的木葡聚糖，得到木葡聚糖低聚糖，并对其进行了分离和表征。其中最丰富的两种化合物为七糖（XXXG）和八糖（XXLG），并通过参考其他结构相关的木葡聚糖化合物的生物活性进行了检测。研究表明，还原的低聚物（XXLGol）可以促进小麦胚囊的生长，而不依赖于2,4-二氯苯氧乙酸（2,4- d）的存在。在2,4- d的存在下，纳摩尔浓度的XXLGol增加了生长素诱导的反应。研究发现，XXLGol是一种信号分子，能够在纳米摩尔浓度下诱导悬液细胞或原生质体中α -l-聚焦酶反应的快速增加，并调节2,4- d或赤霉素酸诱导的α -l-聚焦酶。
Treatment of the xyloglucan isolated from the seeds of Hymenaea courbaril with Humicola insolens endo-1,4-beta-d-glucanase I produced xyloglucan oligosaccharides, which were then isolated and characterized. The two most abundant compounds were the heptasaccharide (XXXG) and the octasaccharide (XXLG), which were examined by reference to the biological activity of other structurally related xyloglucan compounds. The reduced oligomer (XXLGol) was shown to promote growth of wheat (Triticum aestivum) coleoptiles independently of the presence of 2, 4-dichlorophenoxyacetic acid (2,4-D). In the presence of 2,4-D, XXLGol at nanomolar concentrations increased the auxin-induced response. It was found that XXLGol is a signaling molecule, since it has the ability to induce, at nanomolar concentrations, a rapid increase in an alpha-l-fucosidase response in suspended cells or protoplasts of Rubus fruticosus L. and to modulate 2,4-D or gibberellic acid-induced alpha-l-fucosidase.
影响因子：
3.1
作者：
BRACCINI, I;DUPENHOAT, CH;GAGE, DA
通讯作者：
GAGE, DA