Aurora B kinase and protein phosphatase 1 have opposing roles in modulating kinetochore assembly.
Aurora B kinase and protein phosphatase 1 have opposing roles in modulating kinetochore assembly.
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DOI：
10.1083/jcb.200710019
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发表时间：
2008-04-21
期刊：
影响因子：
--
通讯作者：
Stukenberg PT
中科院分区：
文献类型：
--
作者：
Emanuele MJ;Lan W;Jwa M;Miller SA;Chan CS;Stukenberg PT
外动粒结合微管以控制染色体运动。外动粒组装仅限于有丝分裂，而内动粒在整个细胞周期中保持与着丝粒相连。调节这种瞬时组装的线索是未知的。我们发现，抑制极光B激酶显着减少外动粒组件非洲爪蟾和人类组织培养细胞，青蛙卵提取物，和芽殖酵母。在X. leavis M相提取物，预组装的动粒在用药物或抗体抑制Aurora B活性后解体。极光B抑制诱导的动粒解体通过抑制蛋白磷酸酶1（PP 1）活性来拯救。PP1是着丝粒在M期退出时解体所必需的，纯化的酶足以引起分离的有丝分裂核的解体。这些数据表明，极光B活动所需的着丝粒的维护和PP1是必要的，足以拆卸着丝粒。我们认为，极光B和PP 1协调细胞周期依赖性的变化，通过磷酸化的动粒底物的动粒组装。
The outer kinetochore binds microtubules to control chromosome movement. Outer kinetochore assembly is restricted to mitosis, whereas the inner kinetochore remains tethered to centromeres throughout the cell cycle. The cues that regulate this transient assembly are unknown. We find that inhibition of Aurora B kinase significantly reduces outer kinetochore assembly in Xenopus laevis and human tissue culture cells, frog egg extracts, and budding yeast. In X. leavis M phase extracts, preassembled kinetochores disassemble after inhibiting Aurora B activity with either drugs or antibodies. Kinetochore disassembly, induced by Aurora B inhibition, is rescued by restraining protein phosphatase 1 (PP1) activity. PP1 is necessary for kinetochores to disassemble at the exit from M phase, and purified enzyme is sufficient to cause disassembly on isolated mitotic nuclei. These data demonstrate that Aurora B activity is required for kinetochore maintenance and that PP1 is necessary and sufficient to disassemble kinetochores. We suggest that Aurora B and PP1 coordinate cell cycle–dependent changes in kinetochore assembly though phosphorylation of kinetochore substrates.
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DOI：
10.1083/jcb.153.4.865
发表时间：
2001-05-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Adams RR;Maiato H;Earnshaw WC;Carmena M
通讯作者：
Carmena M
小分子黄质蛋白揭示了Aurora B在校正动型微管附着和维持纺锤体组装检查点方面的作用。
DOI：
10.1083/jcb.200208092
发表时间：
2003-04-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hauf S;Cole RW;LaTerra S;Zimmer C;Schnapp G;Walter R;Heckel A;van Meel J;Rieder CL;Peters JM
通讯作者：
Peters JM
影响因子：
9.2
作者：
Famulski, Jakub K.;Chan, Gordon K.
通讯作者：
Chan, Gordon K.
影响因子：
4
作者：
Johnson, VL;Scott, MIF;Taylor, SS
通讯作者：
Taylor, SS
影响因子：
9.2
作者：
Knowlton, Anne Lide;Lan, Weijie;Stukenberg, P. Todd
通讯作者：
Stukenberg, P. Todd