Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation.
Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation.
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DOI：
10.1083/jcb.153.4.865
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发表时间：
2001-05-14
期刊：
影响因子：
--
通讯作者：
Carmena M
中科院分区：
文献类型：
--
作者：
Adams RR;Maiato H;Earnshaw WC;Carmena M
我们已经进行了生化和双链RNA介导的干扰（RNAi）的作用的两个染色体乘客蛋白，内着丝粒蛋白（INCENP）和极光B激酶，在培养的果蝇细胞的分析。INCENP和aurora B的功能是紧密相连的。这两种蛋白在体外相互结合，DmINCENP是DmAurora B在有丝分裂中正确定位所必需的，并作为组蛋白H3激酶发挥作用。DmAurora B是DmINCENP在着丝粒积累和后期转移到纺锤体所必需的。针对任一蛋白质的RNAi显著抑制细胞实现正常中期染色体排列的能力。然而，细胞在有丝分裂中没有被阻断，并且进入异常的后期，其特征在于姐妹动粒分离的缺陷和大量无定形落后染色质的存在。后期A染色体运动似乎正常，但胞质分裂往往失败。DmINCENP和DmAurora B对于驱动蛋白样蛋白Pavarotti（ZEN-4/CHO 1/MKLP 1）在末期正确定位到中间体不是必需的。这些实验揭示了INCENP是极光B激酶功能所必需的，并证实了染色体乘客在有丝分裂中具有重要作用。
We have performed a biochemical and double-stranded RNA-mediated interference (RNAi) analysis of the role of two chromosomal passenger proteins, inner centromere protein (INCENP) and aurora B kinase, in cultured cells of Drosophila melanogaster. INCENP and aurora B function is tightly interlinked. The two proteins bind to each other in vitro, and DmINCENP is required for DmAurora B to localize properly in mitosis and function as a histone H3 kinase. DmAurora B is required for DmINCENP accumulation at centromeres and transfer to the spindle at anaphase. RNAi for either protein dramatically inhibited the ability of cells to achieve a normal metaphase chromosome alignment. Cells were not blocked in mitosis, however, and entered an aberrant anaphase characterized by defects in sister kinetochore disjunction and the presence of large amounts of amorphous lagging chromatin. Anaphase A chromosome movement appeared to be normal, however cytokinesis often failed. DmINCENP and DmAurora B are not required for the correct localization of the kinesin-like protein Pavarotti (ZEN-4/CHO1/MKLP1) to the midbody at telophase. These experiments reveal that INCENP is required for aurora B kinase function and confirm that the chromosomal passengers have essential roles in mitosis.
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影响因子：
7.8
作者：
GOH, PY;KILMARTIN, JV
通讯作者：
KILMARTIN, JV
DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
Clemens, JC;Worby, CA;Dixon, JE
通讯作者：
Dixon, JE
影响因子：
7.8
作者：
Mackay, AM;Ainsztein, AM;Earnshaw, WC
通讯作者：
Earnshaw, WC
影响因子：
19
作者：
Adams, RR;Carmena, M;Earnshaw, WC
通讯作者：
Earnshaw, WC
裂殖酵母中着丝粒和端粒的细胞周期依赖性特定定位和聚类。
影响因子：
7.8
作者：
Funabiki, H;Hagan, I;Uzawa, S;Yanagida, M
通讯作者：
Yanagida, M