Influence of Paternal 252Cf Neutron Exposure on Abnormal Sperm, Embryonal Lethality, and Liver Tumorigenesis in the F1 Offspring of Mice
Influence of Paternal 252Cf Neutron Exposure on Abnormal Sperm, Embryonal Lethality, and Liver Tumorigenesis in the F1 Offspring of Mice
复制标题
父系 252Cf 中子暴露对 F1 代小鼠精子异常、胚胎致死率和肝脏肿瘤发生的影响
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
A. Ito
中科院分区：
文献类型：
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作者：
Hiromitsu Watanabe;Tadateru Takahashi;Juing‐Yi Lee;M. Ohtaki;G. Roy;Y. Ando;Kazumasa Yamada;T. Gotoh;K. Kurisu;N. Fujimoto;Y. Satow;A. Ito
为了确定中子对亲代生殖细胞的遗传损伤是否会导致后代患癌症，进行了实验。用252Cf中子照射7周龄C3H雄性小鼠，剂量分别为0、50、100和200cGy.照射后2周或3个月，雄性小鼠与9周龄处女C57BL雌性小鼠交配。照射后两周，受照射的雄性小鼠精子畸形发生率增加，当它们与未受照射的雌性小鼠交配时，会以剂量依赖的方式导致胚胎死亡。此外，50cGy组雄性小鼠后代的肝脏肿瘤在44只动物中有19只(43.2%)显著增加，与未照射组(1只；3.2%)形成鲜明对比(P<；0.01)。在100cGy组，39只小鼠中有6只(15%)有皮损。照射后3个月，精子畸形率和胚胎致死率均未见明显增加。50cGy组、100cGy组和200cGy组雄性子代肝脏肿瘤发生率分别为6/20(30%)、5/22(23%)和1/19(5%)，与对照组相比差异无统计学意义。结论：252Cf中子照射诱发的肝癌相关性状(S)的遗传传递可能是导致F1代肝脏肿瘤危险性增加的原因。
Experiments were conducted to determine whether neutron‐induced genetic damage in parental germline cells can lead to the development of cancer in the offspring. Seven‐week‐old C3H male mice were irradiated with 252Cf neutrons at a dose of 0, 50, 100, or 200 cGy. Two weeks or 3 months after irradiation, the male mice were mated with virgin 9‐week‐old C57BL females. Two weeks after irradiation, the irradiated male mice showed an increased incidence of sperm abnormalities, which led to embryo lethalities in a dose‐dependent manner when they were mated with unirradiated female mice. Furthermore, liver tumors in male offspring of male mice in the 50 cGy group were significantly increased in 19 of 44 (43.2%) animals, in clear contrast to the unirradiated group (1 of 31; 3.2%) (P < 0.01). In the 100 cGy group, 6 of 39 (15%) mice had lesions. At 3 months after irradiation abnormal sperm and embryonal lethality were not significantly increased. The incidences of liver tumors in male offspring from the 50 cGy, 100 cGy and 200 cGy groups were 6 of 20 (30%), 5 of 22 (23%) and 1 of 19 (5%), respectively, which are not significantly increased compared with the control. It is concluded that increased hepatic tumor risk in the F1 generation may be caused by genetic transmission of hepatoma‐associated trait(s) induced by 252Cf neutron irradiation.
影响因子：
5.1
作者：
Page, John M.;Harrison, Stephen A.
通讯作者：
Harrison, Stephen A.
B6C3F1 小鼠肝癌发生的遗传决定因素。
影响因子：
3.5
作者：
Drinkwater,NR;Hanigan,MH;Kemp,CJ
通讯作者：
Kemp,CJ