Gene Silencing Through CRISPR Interference in Bacteria: Current Advances and Future Prospects.
Gene Silencing Through CRISPR Interference in Bacteria: Current Advances and Future Prospects.
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DOI：
10.3389/fmicb.2021.635227
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发表时间：
2021
影响因子：
5.2
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Zhang R;Xu W;Shao S;Wang Q
功能遗传学筛选是研究生物学过程和基因元件功能注释的重要方法。CRISPR/Cas（规则间隔短回文重复序列/CRISPR相关蛋白）是遗传学家工具箱中的最新工具，使研究人员能够以前所未有的轻松，准确和高通量编辑基因组。最近，CRISPR干扰（CRISPRi）已被开发为一种新兴技术，该技术利用无催化活性的Cas9（dCas 9）和单向导RNA（sgRNA）来抑制序列特异性基因。本文综述了CRISPRi系统的可编程性、高效性和特异性等特点。此外，我们展示了它在功能遗传筛选中的应用，并强调了它在剖析发病机制方面的潜力。CRISPRi系统的最新发展将为发现细菌中功能重要的基因提供高通量，实用和有效的工具。
Functional genetic screening is an important method that has been widely used to explore the biological processes and functional annotation of genetic elements. CRISPR/Cas (Clustered regularly interspaced short palindromic repeat sequences/CRISPR-associated protein) is the newest tool in the geneticist’s toolbox, allowing researchers to edit a genome with unprecedented ease, accuracy, and high-throughput. Most recently, CRISPR interference (CRISPRi) has been developed as an emerging technology that exploits the catalytically inactive Cas9 (dCas9) and single-guide RNA (sgRNA) to repress sequence-specific genes. In this review, we summarized the characteristics of the CRISPRi system, such as programmable, highly efficient, and specific. Moreover, we demonstrated its applications in functional genetic screening and highlighted its potential to dissect the underlying mechanism of pathogenesis. The recent development of the CRISPRi system will provide a high-throughput, practical, and efficient tool for the discovery of functionally important genes in bacteria.
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