LRIG1 expression and colorectal cancer prognosis.
LRIG1 expression and colorectal cancer prognosis.
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DOI：
10.1186/s12920-020-00846-2
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发表时间：
2021-01-18
影响因子：
2.7
通讯作者：
Majidizadeh T
中科院分区：
文献类型：
--
作者：
Bakherad M;Salimi M;Angaji SA;Mahjoubi F;Majidizadeh T
为了对结直肠癌（CRC）患者做出正确的治疗决策，需要可靠的预测和预后数据。然而，在许多情况下，这些数据是不够的。一些研究表明LRIG 1基因（富含亮氨酸的重复序列和免疫球蛋白样结构域1）在不同类型的癌症中具有预后意义。回顾性分析了102例结直肠癌患者LRIG 1在mRNA和蛋白水平的表达。采用SYBR绿色实时荧光定量RT-PCR技术分析mRNA表达，以甘油醛-3-磷酸脱氢酶（GAPDH）基因为参考基因进行数据标准化。LRIG 1蛋白表达采用免疫组化法进行分析。此外，使用适当的统计分析来评估测试组和对照组中LRIG 1的表达。采用单因素和多因素分析LRIG 1表达的预后意义。结果显示，LRIG 1在大肠癌组织中的表达水平在mRNA和蛋白水平上均下调（P < 0.01），但在大肠癌中不是临床相关的预后指标。因此，它建议，LRIG 1表达分析可能不会被认为是一个重要的问题时，作出明智的和个性化的临床决策有关的管理结直肠癌患者。
To make the right treatment decisions about colorectal cancer (CRC) patients reliable predictive and prognostic data are needed. However, in many cases this data is not enough. Some studies suggest that LRIG1 gene (leucine-rich repeats and immunoglobulin-like domains1) has prognostic implications in different kinds of cancers. One hundred and two patients with colorectal cancer were retrospectively analyzed for LRIG1 expression at both mRNA and protein levels. SYBR Green Real-Time RT-PCR technique was used for mRNA expression analyses and Glyceraldehyde-3-Phosphate Dehydrogenase gene (GAPDH) was considered as a reference gene for data normalization. LRIG1 protein expression was analyzed using Immunohistochemistry. Additionally, appropriate statistic analyses were used to assess the expression of LRIG1 in test and control groups. The prognostic significance of LRIG1 expression was analyzed using the univariate and multivariate analyses. The data revealed that the expression of LRIG1 in both mRNA and protein levels was down regulated in colorectal tumor tissues (P < 0.01) but is not clinically relevant prognostic indicator in CRC. Therefore, it is suggested that LRIG1 expression analyses may not be considered as an important issue when making informed and individualized clinical decisions regarding the management of colorectal cancer patients.
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影响因子：
8.8
作者：
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通讯作者：
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