Soluble CD14 levels reflect liver inflammation in patients with nonalcoholic steatohepatitis.
Soluble CD14 levels reflect liver inflammation in patients with nonalcoholic steatohepatitis.
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DOI：
10.1371/journal.pone.0065211
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nakajima A
中科院分区：
文献类型：
--
作者：
Ogawa Y;Imajo K;Yoneda M;Kessoku T;Tomeno W;Shinohara Y;Kato S;Mawatari H;Nozaki Y;Fujita K;Kirikoshi H;Maeda S;Saito S;Wada K;Nakajima A
肝脏炎症是非酒精性脂肪性肝病（NAFLD）进展的危险因素。然而，肝脏炎症的诊断是非常困难的，并且侵入性肝活检仍然是可靠地检测肝脏炎症的唯一方法。我们以前报道过枯否细胞中CD14的过度表达可能通过对低剂量脂多糖的高反应性后的肝脏炎症引发非酒精性脂肪性肝炎（NASH）的进展。因此，本研究的目的是探讨可溶性CD 14（sCD 14）与NAFLD患者的组织学特征之间的关系。我们的队列包括113名肝活检证实的NAFLD患者和21名年龄匹配的健康对照。血清sCD14水平测定采用酶联免疫吸附试验。血清sCD14水平与NASH的诊断显著相关，区分非NASH和NASH的受试者工作特征曲线（AUROC）下的面积为0.802。此外，根据NAFLD活动评分和肝脏CD 14 mRNA表达（与NAFLD患者的膜CD 14（mCD 14）表达相关），血清sCD 14水平与疾病活动性显着相关。多元回归分析显示，血清sCD 14水平与肝脏炎症独立相关。区分NAFLD患者轻度和重度肝脏炎症的AUROC为0.752。我们发现血清sCD 14水平随着NAFLD患者肝脏炎症程度的增加而显著增加，反映了肝脏CD 14表达的增加。血清sCD 14是预测肝脏炎症恶化的一个有前途的工具，并可能为评估NAFLD的治疗效果提供潜在的生物标志物。
Liver inflammation is a risk factor for the progression of nonalcoholic fatty liver disease (NAFLD). However, the diagnosis of liver inflammation is very difficult and invasive liver biopsy is still the only method to reliably detect liver inflammation. We previously reported that overexpression of CD14 in Kupffer cells may trigger the progression to nonalcoholic steatohepatitis (NASH) via liver inflammation following hyper-reactivity to low-dose lipopolysaccharide. Therefore, the aim of this study was to investigate the relationship between soluble type of CD14 (sCD14) and histological features in patients with NAFLD. Our cohort consisted of 113 patients with liver biopsy-confirmed NAFLD and 21 age-matched healthy controls. Serum sCD14 levels were measured by an enzyme-linked immunosorbent assay. Serum sCD14 levels were significantly associated with diagnosis of NASH and the area under the receiver operator characteristic curve (AUROC) to distinguish between not NASH and NASH was 0.802. Moreover, serum sCD14 levels were significantly associated with the disease activity based on NAFLD activity score and hepatic CD14 mRNA expression, which is correlated with membrane CD14 (mCD14) expression, in patients with NAFLD. In multiple regression analysis, the serum sCD14 levels were independently associated with liver inflammation. The AUROC to distinguish between mild and severe liver inflammation in patients with NAFLD was 0.752. We found that serum sCD14 levels increased significantly with increasing liver inflammation grade in patients with NAFLD, reflecting increased hepatic CD14 expression. Serum sCD14 is a promising tool to predict the worsening of liver inflammation, and may offer a potential biomarker for evaluation of therapeutic effects in NAFLD.
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影响因子：
13.5
作者：
POWELL, EE;COOKSLEY, WGE;POWELL, LW
通讯作者：
POWELL, LW
影响因子：
13.5
作者：
Bedossa, P;Dargère, D;Paradis, V
通讯作者：
Paradis, V
影响因子：
0.9
作者：
Luan, X.;Liu, Y.;Li, M.
通讯作者：
Li, M.
影响因子：
32.4
作者：
PUGIN, J;HEUMANN, D;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
DOI：
10.1186/1476-9255-7-15
发表时间：
2010-03-30
期刊：
Journal of inflammation (London, England)
影响因子：
--
作者：
Harte AL;da Silva NF;Creely SJ;McGee KC;Billyard T;Youssef-Elabd EM;Tripathi G;Ashour E;Abdalla MS;Sharada HM;Amin AI;Burt AD;Kumar S;Day CP;McTernan PG
通讯作者：
McTernan PG