Expression patterns of CEACAM5 and CEACAM6 in primary and metastatic cancers.
Expression patterns of CEACAM5 and CEACAM6 in primary and metastatic cancers.
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DOI：
10.1186/1471-2407-7-2
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发表时间：
2007-01-03
期刊：
影响因子：
3.8
通讯作者：
Goldenberg DM
中科院分区：
文献类型：
--
作者：
Blumenthal RD;Leon E;Hansen HJ;Goldenberg DM
许多乳腺癌、胰腺癌、结肠癌和非小细胞肺癌细胞系表达CEACAM 6（NCA-90）和CEACAM 5（癌胚抗原，CEA），并且针对这两者的抗体可以在体外和体内影响肿瘤细胞生长。在这里，我们比较了两种抗原作为乳腺癌，胰腺癌，肺癌，卵巢癌和前列腺癌，包括患者匹配的正常，原发性肿瘤，转移性乳腺癌和结肠癌标本的组织学表型的函数。使用组织微阵列通过免疫组织化学（IHC）测定抗原表达，其中MN-15和MN-3抗体分别靶向CEACAM 6的A1 B1-和N-结构域，MN-14抗体靶向CEACAM 5的A3 B3结构域。使用抗生物素蛋白-生物素-二氨基联苯染色进行IHC。记录每种组织型的平均评分± SD（0 =阴性/8 =最高）。在所有肿瘤中，CEACAM 6的表达量均大于CEACAM 5的表达量，并反映了肿瘤的组织类型。在乳腺肿瘤中，CEACAM 6在乳头状>浸润性导管>小叶>叶状肿瘤中最高;在胰腺肿瘤中，中分化>高分化>低分化肿瘤;粘液性卵巢腺癌的CEACAM 6几乎是浆液性卵巢腺癌的3倍;肺腺癌>鳞状肿瘤;结肠癌肝转移>原发肿瘤=淋巴结转移>正常肠。然而，CEACAM 6在前列腺癌和正常组织中的表达相似。在肝脏中发现的转移性结肠肿瘤中CEACAM 6的量高于许多原发性结肠肿瘤。相比之下，乳腺、结肠或肺肿瘤淋巴结转移的CEACAM 6免疫染色与原发肿瘤相似。CEACAM 6表达在许多实体瘤中升高，但作为组织型的函数是可变的。基于证明CEACAM 6在肿瘤细胞迁移、侵袭和粘附以及远处转移形成中的作用的先前工作（布卢门塔尔等人，Cancer Res 65：8809-8817，2005），它可能是基于抗体的治疗的有希望的靶标。
Many breast, pancreatic, colonic and non-small-cell lung carcinoma lines express CEACAM6 (NCA-90) and CEACAM5 (carcinoembryonic antigen, CEA), and antibodies to both can affect tumor cell growth in vitro and in vivo. Here, we compare both antigens as a function of histological phenotype in breast, pancreatic, lung, ovarian, and prostatic cancers, including patient-matched normal, primary tumor, and metastatic breast and colonic cancer specimens. Antigen expression was determined by immunohistochemistry (IHC) using tissue microarrays with MN-15 and MN-3 antibodies targeting the A1B1- and N-domains of CEACAM6, respectively, and the MN-14 antibody targeting the A3B3 domain of CEACAM5. IHC was performed using avidin-biotin-diaminobenzide staining. The average score ± SD (0 = negative/8 = highest) for each histotype was recorded. For all tumors, the amount of CEACAM6 expressed was greater than that of CEACAM5, and reflected tumor histotype. In breast tumors, CEACAM6 was highest in papillary > infiltrating ductal > lobular > phyllodes; in pancreatic tumors, moderately-differentiated > well-differentiated > poorly-differentiated tumors; mucinous ovarian adenocarcinomas had almost 3-fold more CEACAM6 than serous ovarian adenocarcinomas; lung adenocarcinomas > squamous tumors; and liver metastases of colonic carcinoma > primary tumors = lymph nodes metastases > normal intestine. However, CEACAM6 expression was similar in prostate cancer and normal tissues. The amount of CEACAM6 in metastatic colon tumors found in liver was higher than in many primary colon tumors. In contrast, CEACAM6 immunostaining of lymph node metastases from breast, colon, or lung tumors was similar to the primary tumor. CEACAM6 expression is elevated in many solid tumors, but variable as a function of histotype. Based on previous work demonstrating a role for CEACAM6 in tumor cell migration, invasion and adhesion, and formation of distant metastases (Blumenthal et al., Cancer Res 65: 8809–8817, 2005), it may be a promising target for antibody-based therapy.
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影响因子：
8
作者：
Duxbury, MS;Ito, H;Whang, EE
通讯作者：
Whang, EE
影响因子：
11.2
作者：
Duxbury, MS;Ito, H;Whang, EE
通讯作者：
Whang, EE
DOI：
10.1084/jem.122.3.467
发表时间：
1965-09-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gold P;Freedman SO
通讯作者：
Freedman SO
影响因子：
6.3
作者：
Hinoda, Y;Saito, T;Imai, K
通讯作者：
Imai, K
影响因子：
11.2
作者：
Blumenthal, RD;Hansen, HJ;Goldenberg, DM
通讯作者：
Goldenberg, DM