Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome.
Deploying an In Vitro Gut Model to Assay the Impact of the Mannan-Oligosaccharide Prebiotic Bio-Mos on the Atlantic Salmon (Salmo salar) Gut Microbiome.
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DOI：
10.1128/spectrum.01953-21
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发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
Llewellyn, Martin S.
中科院分区：
文献类型：
--
作者：
Kazlauskaite, Raminta;Cheaib, Bachar;Humble, Joseph;Heys, Chloe;Ijaz, Umer Zeeshan;Connelly, Stephanie;Sloan, William T.;Russell, Julie;Martinez-Rubio, Laura;Sweetman, John;Kitts, Alex;McGinnity, Philip;Lyons, Philip;Llewellyn, Martin S.
α-甘露寡糖 (MOS) 益生元作为免疫调节剂广泛应用于动物农业，并可促进生长和肠道健康。它们的作用方式被认为是通过对宿主微生物群落及其相关代谢的影响来介导的。 Bio-Mos 是一种目前用于农业饲料行业的市售益生元，但研究显示其对鱼类性能和饲料效率的影响结果截然不同。因此，需要进行详细的研究来调查 MOS 补充剂对鱼类微生物群的影响，以增强我们对 MOS 与肠道健康之间联系的理解。为了评估 Bio-Mos 在鲑鱼水产养殖中作为益生元生长促进剂的潜在用途，我们修改了已建立的大西洋鲑鱼体外肠道模型 SalmoSim，以评估其对宿主微生物群落的影响。从四条成年养殖鲑鱼的盲肠室中获得的微生物群落被接种到 SalmoSim 的生物三重反应器中。补充益生元治疗 20 天，然后是 6 天的清除期。在培养基中添加 Bio-Mos 会导致甲酸盐 (P = 0.001)、丙酸盐 (P = 0.037) 和 3-甲基丁酸 (P = 0.024) 水平显着增加，这与几种主要是厌氧微生物属（梭杆菌属、Agarivorans、假交替单胞菌属）丰度的增加相关。使用 16S rDNA 标记的 DNA 元条形码证实了微生物群落组成因补充 Bio-Mos 而发生显着变化，并观察到产生乳酸的肉杆菌有所增加。结合之前的体内研究，将增强的挥发性脂肪酸产量与 MOS 补充剂与宿主生长和性能联系起来，我们的数据表明 Bio-Mos 可能在鲑鱼生产中具有价值。此外，我们的数据强调了体外肠道模型对微生物组调节剂体内试验的补充的潜在作用。重要性在本文中，我们使用大西洋鲑鱼肠道微生物环境的体外模拟器报告了益生元（α-MOS 补充剂）对微生物群落的影响结果。我们的数据表明，Bio-Mos 在鲑鱼生产中可能具有价值，因为它可以提高鲑鱼幽门盲肠微生物群的挥发性脂肪酸产量，并与微生物群落组成的显着变化以及观察到的产乳酸肉杆菌的增加相关。结合之前的体内研究，将增强的挥发性脂肪酸产量与 MOS 补充剂与宿主生长和性能联系起来，我们的数据表明 Bio-Mos 可能在鲑鱼生产中具有价值。此外，我们的数据强调了体外肠道模型在增强微生物组调节剂体内试验方面的潜在作用。
Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a commercially available prebiotic currently used in the agri-feed industry, but studies show contrasting results of its effect on fish performance and feed efficiency. Thus, detailed studies are needed to investigate the effect of MOS supplements on the fish microbiome to enhance our understanding of the link between MOS and gut health. To assess Bio-Mos for potential use as a prebiotic growth promoter in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. The microbial communities obtained from ceca compartments from four adult farmed salmon were inoculated in biological triplicate reactors in SalmoSim. Prebiotic treatment was supplemented for 20 days, followed by a 6-day washout period. Inclusion of Bio-Mos in the media resulted in a significant increase in formate (P = 0.001), propionate (P = 0.037) and 3-methyl butanoic acid (P = 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to Bio-Mos supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to complementin vivo trials of microbiome modulators. IMPORTANCE In this paper we report the results of the impact of a prebiotic (alpha-MOS supplementation) on microbial communities, using an in vitro simulator of the gut microbial environment of the Atlantic salmon. Our data suggest that Bio-Mos may be of value in salmonid production as it enhances volatile fatty acid production by the microbiota from salmon pyloric ceca and correlates with a significant shift in microbial community composition with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to augment in vivo trials of microbiome modulators.
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影响因子：
4.1
作者：
Kennedy, David A.;Kurath, Gael;Wargo, Andrew R.
通讯作者：
Wargo, Andrew R.
DOI：
10.5713/ajas.2012.12414
发表时间：
2013-01-01
期刊：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
影响因子：
--
作者：
Ao, Z.;Choct, M.
通讯作者：
Choct, M.
影响因子：
2
作者：
da Silva, Bruno Correa;Vieira, Felipe do Nascimento;Seiffert, Walter Quadros
通讯作者：
Seiffert, Walter Quadros
影响因子：
0.6
作者：
Gurbuz, Emel;Inal, Fatma;Kucukkaya, Fatih
通讯作者：
Kucukkaya, Fatih
影响因子：
2.4
作者：
Adams, C. E.;Turnbull, J. F.;Huntingford, F. A.
通讯作者：
Huntingford, F. A.