The cyclin-dependent kinase G group defines a thermo-sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU2AF65A.
The cyclin-dependent kinase G group defines a thermo-sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU2AF65A.
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DOI：
10.1111/tpj.13914
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Doonan JH
中科院分区：
文献类型：
--
作者：
Cavallari N;Nibau C;Fuchs A;Dadarou D;Barta A;Doonan JH
使生长和发育适应温度变化的能力对于产生对预测的温度变化有弹性的植物品种至关重要。然而，植物对温度逐步升高的反应机制才刚刚开始阐明。在这里，我们报告说，细胞周期蛋白依赖性激酶G1（CDKG1）是一个温度敏感的mRNA剪接级联反应的核心元件，它将环境温度的变化转化为基本剪接体组分ATU2AF65A的差异表达。CDKG1以温度依赖性方式选择性剪接。我们发现这个过程部分依赖于细胞周期蛋白依赖性激酶G2（CDKG 2）和相互作用的辅因子细胞周期蛋白L1（CYCL1），导致两种不同的信使RNA。两种CDKG1转录物的相对丰度与环境温度相关，并且可能与相关蛋白质同种型的不同表达水平相关。两种CDKG1替代转录物都是在整个温度范围内完全补充ATU2AF65A表达所必需的。我们的数据支持一种先前未确定的温度依赖性机制，该机制基于CDKG1的选择性剪接（AS）并受CDKG2和CYCL1的调节。我们建议，环境温度的变化影响CDKG1转录本的相对丰度，这反过来又转化为差异CDKG1蛋白表达协调ATU2AF65A的AS。现代农业最重要的挑战之一是生产更好地适应预测的气候变化的植物品种。然而，对植物对环境温度反应的深入了解仍然不完整。在这里，我们描述了拟南芥细胞周期蛋白依赖性激酶家族中的一个基因回路，该基因回路能够通过调节mRNA加工以适应环境温度来调节下游靶标的活性。
The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the cyclin‐dependent kinase G1 (CDKG1) is a central element in a thermo‐sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature‐dependent manner. We found that this process is partly dependent on both the cyclin‐dependent kinase G2 (CDKG2) and the interacting co‐factor CYCLIN L1 (CYCL1), resulting in two distinct messenger RNAs. The relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature‐dependent mechanism based on the alternative splicing (AS) of CDKG1 and regulated by CDKG2 and CYCL1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts, and this in turn translates into differential CDKG1 protein expression coordinating the AS of ATU2AF65A. One of the most important challenges of modern agriculture is the production of plant varieties that are better adapted to the predicted climatic changes. However, a deep understanding of the ambient temperature response in plants remains incomplete. Here we describe a gene circuit in the cyclin‐dependent kinase family of Arabidopsis able to modulate the activity of downstream targets by adjusting mRNA processing to ambient temperature.
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