The equine alveolar macrophage: functional and phenotypic comparisons with peritoneal macrophages.
The equine alveolar macrophage: functional and phenotypic comparisons with peritoneal macrophages.
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DOI：
10.1016/j.vetimm.2013.07.003
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发表时间：
2013-10-01
影响因子：
1.8
通讯作者：
Pirie, Scott R.
中科院分区：
文献类型：
--
作者：
Karagianni, Anna E.;Kapetanovic, Ronan;McGorum, Bruce C.;Hume, David A.;Pirie, Scott R.
肺泡巨噬细胞(AM)是所有物种肺部的第一道防线，在调节对吸入病原体的免疫反应中起着至关重要的作用。对AM的功能和表型的详细了解是阐明它们在防止下呼吸道机会性细菌定植中的作用和制定适当的预防策略的必要先决条件。这项研究的目的是通过与腹膜巨噬细胞(PM)进行功能和表型比较，在组织水平上表征这种重要的先天免疫细胞。我们假设起源的组织决定了AM的独特表型，这可能构成某些马呼吸道疾病的合适的治疗靶点。用不同的Toll样受体配体刺激9匹马肺和腹膜巨噬细胞，分别用Griess反应、酶联免疫吸附试验和/或定量聚合酶链式反应测定细胞产生亚硝酸盐、肿瘤坏死因子α、白介素10和吲哚胺2，3-双加氧酶。在吞噬能力和几种细胞表面标志物表达的基础上对细胞进行比较。内毒素、多聚肌胞苷多胞酸(Poly IC)和热灭活鼠伤寒沙门氏菌刺激后，AM的肿瘤坏死因子α释放增加，而PM则无明显变化；AM高表达CD14、CD163和TLR4等巨噬细胞标志物，而PMS仅高表达TLR4。AM，而不是PM，显示出有效的吞噬活性。我们的结果表明，在不同的促炎配体的刺激下，AM比PM更活跃，从而支持了局部微环境在巨噬细胞激活状态中的重要性。这些信息为我们更好地了解巨噬细胞及其微环境在马先天免疫中的作用提供了有价值的知识基础。
Alveolar macrophages (AMs) constitute the first line of defence in the lung of all species, playing a crucial role in the regulation of immune responses to inhaled pathogens. A detailed understanding of the function and phenotype of AMs is a necessary pre-requisite to both elucidating their role in preventing opportunistic bacterial colonisation of the lower respiratory tract and developing appropriate preventative strategies. The purpose of the study was to characterise this important innate immune cell at the tissue level by making functional and phenotypic comparisons with peritoneal macrophages (PMs). We hypothesised that the tissue of origin determines a unique phenotype of AMs, which may constitute an appropriate therapeutic target for certain equine respiratory diseases. Macrophages isolated from the lung and the peritoneal cavity of 9 horses were stimulated with various toll like receptor (TLR) ligands and the production of nitrite, tumour necrosis factor alpha (TNFα), interleukin (IL) 10 and indoleamine 2,3-dioxygenase (IDO) were measured by the Griess reaction and enzyme linked immunosorbent assay (ELISA) and/or quantitative polymerase chain reaction, respectively. Cells were also compared on the basis of phagocytic-capacity and the expression of several cell surface markers. AMs, but not PMs, demonstrated increased TNFα release following stimulation with LPS, polyinosinic polycytidylic acid (Poly IC) and heat-killed Salmonella typhinurium and increased TNFα and IDO mRNA expression when stimulated with LPS. AMs showed high expression of the specific macrophage markers cluster of differentiation (CD) 14, CD163 and TLR4, whereas PMs showed high expression of TLR4 only. AMs, but not PMs, demonstrated efficient phagocytic activity. Our results demonstrate that AMs are more active than PMs when stimulated with various pro-inflammatory ligands, thus supporting the importance of the local microenvironment in the activation status of the macrophage. This information provides a valuable knowledge base on which to improve our understanding of the role of macrophages and their microenvironment in equine innate immunity.
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影响因子：
0.9
作者：
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通讯作者：
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影响因子：
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通讯作者：
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