Gentle organ manipulation during harvest as a key determinant of survival of fatty livers after transplantation in the rat
Gentle organ manipulation during harvest as a key determinant of survival of fatty livers after transplantation in the rat
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收获过程中温和的器官操作是大鼠移植后脂肪肝存活的关键决定因素
DOI：
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发表时间：
1999
影响因子：
3.1
通讯作者：
Ronald G. Thurman
中科院分区：
文献类型：
--
作者：
P. Schemmer;R. Schoonhoven;James A. Swenberg;H. Bunzendahl;James A. Raleigh;J. Lemasters;Ronald G. Thurman
摘取过程中的原位器官操作和脂肪变性都通过与库普弗细胞有关的机制降低肝移植后的存活率；因此，本文比较了它们对生存的影响。单剂量乙醇诱导Lewis大鼠中度脂肪变性，而长期给药乙醇诱导供鼠重度脂肪变性。在前12分钟进行最小剥离后，轻轻地操作肝脏或随后单独放置13分钟。在UW溶液中冷藏1h后进行原位肝移植。乙醇使肝脏脂质含量增加到中度或重度脂肪变性水平，使移植后存活率从100%降低到约70% （P < 0.05）。然而，温和的操作使正常、盐水处理的大鼠和喂食高脂肪对照饮食的大鼠肝脏的存活率下降到约30% （P < 0.05）。此外，在短期或长期使用乙醇后，脂肪肝操作使存活率从70%降低到约13% （P < 0.05）。此外，在脂肪移植物移植后，操作显著提高血清转氨酶、总胆红素和坏死约2- 20倍。在收获结束时，通过操纵脂肪移植物，台盼蓝分布时间和2-硝基咪唑结合评估的缺氧显著提高了约2至4倍。氯化钆，一种库普弗细胞毒物，阻止了操作的有害影响。这些数据首次证明，虽然脂肪变性对生存有害，但在原发性无功能机制中，器官操纵比脂肪起着更大的作用。
Abstract Both in situ organ manipulation during harvest and steatosis reduce survival after liver transplantation via mechanisms involving Kupffer cells; thus, their effect on survival was compared here. Moderate steatosis was induced by a single dose of ethanol to Lewis rats, while long-term administration of ethanol yielded severe steatosis in donor animals. After minimal dissection during the first 12 min, livers were either manipulated gently or left alone for 13 min subsequently. Orthotopic liver transplantation was performed after 1 h of cold storage in UW solution. Ethanol increased hepatic lipid content to a level of moderate or severe steatosis that reduced survival after transplantation from 100 % to approximately 70 % (P < 0.05). However, gentle manipulation decreased survival to approximately 30 % (P < 0.05) in livers from normal, saline-treated rats and in livers from rats fed a high-fat control diet. Moreover, after short- or long-term ethanol administration, manipulation of fatty livers decreased survival from 70 % to approximately 13 % (P < 0.05). Further, manipulation elevated serum transaminases, total bilirubin, and necrosis significantly about 2- to 20-fold in fatty grafts after transplantation. At the end of harvest, trypan blue distribution time and hypoxia assessed from 2-nitroimidazole binding were elevated significantly about two- to fourfold by manipulation of fatty grafts. Gadolinium chloride, a Kupffer cell toxicant, blocked the detrimental effects of manipulation. These data demonstrate for the first time that, while steatosis is detrimental for survival, organ manipulation plays a much greater role than fat in mechanisms of primary nonfunction.
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乙醇处理的大鼠脂肪肝移植后，库普弗细胞的破坏可增加存活率并减少移植物损伤。
DOI：
10.1002/lt.500020509
发表时间：
1996
期刊：
Liver transplantation and surgery : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society.
影响因子：
--
作者：
Zhong,Z;Connor,H;Mason,RP;Qu,W;Stachlewitz,RF;Gao,W;Lemasters,JJ;Thurman,RG
通讯作者：
Thurman,RG
影响因子：
29.4
作者：
ADACHI, Y;MOORE, LE;THURMAN, RG
通讯作者：
THURMAN, RG
DOI：
10.1111/j.1530-0277.1991.tb00563.x
发表时间：
1991-08-01
影响因子：
3.2
作者：
LIEBER, CS
通讯作者：
LIEBER, CS
库普弗细胞失活可最大限度地减少乙醇处理大鼠脂肪肝脏的再灌注损伤。
DOI：
--
发表时间：
1995
期刊：
Transplantation proceedings.
影响因子：
--
作者：
Zhong,Z;Qu,W;Connor,HD;Thurman,RG
通讯作者：
Thurman,RG
影响因子：
29.4
作者：
Enomoto, N;Ikejima, K;Thurman, RG
通讯作者：
Thurman, RG