Early monitoring antiangiogenesis treatment response of Sunitinib in U87MG Tumor Xenograft by (18)F-FLT MicroPET/CT imaging.
Early monitoring antiangiogenesis treatment response of Sunitinib in U87MG Tumor Xenograft by (18)F-FLT MicroPET/CT imaging.
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通过 18F-FLT MicroPET/CT 成像早期监测舒尼替尼在 U87MG 肿瘤异种移植物中的抗血管生成治疗反应
DOI：
10.1155/2014/218578
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发表时间：
2014
影响因子：
--
通讯作者：
Zhang YJ
中科院分区：
文献类型：
--
作者：
Bao X;Wang MW;Zhang YP;Zhang YJ
瞄准。本研究旨在通过18F-Flt microPET/CT纵向显像监测苏尼替尼对U87 MG多形性胶质母细胞瘤模型的早期治疗反应。方法：研究方法。给U87 MG荷瘤小鼠灌胃给予苏尼替尼80 mg/kg，连续7d。分别于治疗后第0、1、3、7、13天进行18F-Flt microPET/CT扫描。测量肿瘤大小和体重。分别用抗Ki67抗体和抗CD31抗体对肿瘤标本进行增殖和微血管密度(MVD)免疫组织化学分析。结果。苏尼替尼组肿瘤对健侧肌肉的18F-Flt摄取比值(T/M)从基线至第3天逐渐下降(T/M_0=2.98±0.33；T/M_3=2.23±0.36；P<0.001)，第7天降至最低点(T/M_7=1.96±0.35；P<；0.001)，第13天恢复。肿瘤的18F-Flt摄取量与Ki67染色指数和MVD相关。结论。通过18F-Flt PET可以预测对Sunitinib的早期治疗反应，这将有助于监测抗血管生成治疗。
Aim. It was aimed to monitor early treatment response of Sunitinib in U87MG models mimicking glioblastoma multiforme by longitudinal 18F-FLT microPET/CT imaging in this study. Methods. U87MG tumor mice were intragastrically injected with Sunitinib at a dose of 80 mg/kg for consecutive 7 days. 18F-FLT microPET/CT scans were acquired on days 0, 1, 3, 7, and 13 after therapy. Tumor sizes and body weight were measured. Tumor samples were collected for immunohistochemical analysis of proliferation and microvessel density (MVD) with anti-Ki67 and anti-CD31, respectively. Results. The uptake ratios of tumor to the contralateral muscle (T/M) of 18F-FLT in the Sunitinib group decreased from baseline to day 3 (T/M0 = 2.98 ± 0.33; T/M3 = 2.23 ± 0.36; P < 0.001), reached the bottom on day 7 (T/M7 = 1.96 ± 0.35; P < 0.001), and then recovered on day 13. The T/M of 18F-FLT uptake in the control group remained around 3.0. There was no difference for the tumor size between both groups until day 11. 18F-FLT uptakes of tumor were correlated with Ki67 staining index and MVD. Conclusion. Early therapy response to Sunitinib could be predicted via 18F-FLT PET, which will contribute to monitoring antiangiogenesis treatment.
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DOI：
10.1158/1078-0432.ccr-11-1677
发表时间：
2011-12-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Liu G;Jeraj R;Vanderhoek M;Perlman S;Kolesar J;Harrison M;Simoncic U;Eickhoff J;Carmichael L;Chao B;Marnocha R;Ivy P;Wilding G
通讯作者：
Wilding G
影响因子：
3.1
作者：
Perumal, Meg;Stronach, Euan A.;Aboagye, Eric O.
通讯作者：
Aboagye, Eric O.
影响因子：
2.3
作者：
Semrad TJ;Eddings C;Dutia MP;Christensen S;Lara PN Jr
通讯作者：
Lara PN Jr
DOI：
10.1097/ppo.0b013e3182431c6f
发表时间：
2012-01
期刊：
Cancer journal (Sudbury, Mass.)
影响因子：
--
作者：
Gerstner ER;Batchelor TT
通讯作者：
Batchelor TT
影响因子：
9.3
作者：
Goggi, Julian L.;Bejot, Romain;Bhakoo, Kishore K.
通讯作者：
Bhakoo, Kishore K.