Immunoregulatory activity of decidua in spontaneous early pregnancy loss.
Immunoregulatory activity of decidua in spontaneous early pregnancy loss.
复制标题
自发性早期妊娠流产中蜕膜的免疫调节活性。
作者：
N. Vassiliadou;R. Searle;Judith N. Bulmer
本研究旨在探讨与表面上正常的生育女性相比，自然流产女性的蜕膜内分泌的分子的免疫调节特性是否发生了改变。分离来自 22 名接受治疗性妊娠终止的妇女和 25 名经历散发性自发性早期妊娠流产的妇女的未分级蜕膜细胞，培养 24 小时和 72 小时，并收集上清液。研究了蜕膜上清液对植物血凝素（PHA）诱导的外周血淋巴细胞增殖的影响。 91% 的治疗性流产病例的 24 小时细胞培养上清液中检测到免疫抑制活性，而自然流产样本中只有 64% 的检测到免疫抑制活性；所有治疗性流产样本和 90% 自然流产病例的 72 小时上清液抑制了淋巴细胞增殖。其余自然流产样本（24小时上清液中的36％；72小时上清液中的10％）对淋巴细胞增殖有增强或没有影响。在治疗性流产样品中未观察到淋巴细胞增殖的增强，并且对于 50% 浓度的 24 小时蜕膜细胞上清液，刺激细胞增殖与自然流产之间存在显着相关性（P = 0.02）。这些发现表明，在经历自发性早期妊娠流产的女性亚组中，蜕膜内的可溶性因子表现出改变的免疫反应，这可能与胎儿排斥的复杂过程有关。
The present study aimed to address whether the immunoregulatory properties of the molecules secreted within decidua were altered in women suffering spontaneous miscarriage, compared with apparently normal fertile women. Unfractionated decidual cells from 22 women undergoing therapeutic pregnancy terminations and 25 women experiencing a sporadic spontaneous early pregnancy loss were isolated, cultured for 24 h and 72 h, and supernatants were collected. The effect of decidual supernatants on phytohaemagglutinin (PHA)-induced peripheral blood lymphocyte proliferation was investigated. Immunosuppressive activity was detected in 24 h cell culture supernatants from 91% of therapeutic abortion cases compared with only 64% of spontaneous abortion samples; 72 h supernatants from all of therapeutic abortion samples and 90% of spontaneous abortion cases suppressed lymphoproliferation. The remaining spontaneous abortion samples (36% of 24 h supernatants; 10% of 72 h supernatants) enhanced or had no effect on lymphocyte proliferation. Enhancement of lymphocyte proliferation was not observed in therapeutic abortion samples, and the association between stimulation of cell proliferation and spontaneous abortion was significant for 24 h decidual cell supernatants at 50% concentration (P = 0.02). These findings suggest that in a subgroup of women experiencing spontaneous early pregnancy loss, soluble factors within decidua display altered immune responses that may be implicated in the complex process of fetal rejection.
对回忆抗原的增殖反应与有反复自然流产史的妇女的妊娠结局相关。
DOI：
10.1172/jci119652
发表时间：
1997
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Bermas,BL;Hill,JA
通讯作者：
Hill,JA