AFM and ellipsometric studies on LB films of natural asymmetric and symmetric bolaamphiphilic archaebacterial tetraether lipids on silicon wafers.

AFM and ellipsometric studies on LB films of natural asymmetric and symmetric bolaamphiphilic archaebacterial tetraether lipids on silicon wafers.
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硅片上天然不对称和对称双亲性古细菌四醚脂质 LB 膜的 AFM 和椭圆测量研究

DOI:
10.1016/j.colsurfb.2010.03.015
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发表时间:
2010
期刊:
Colloids and surfaces. B, Biointerfaces
影响因子:
--
通讯作者:
Bakowsky U.
Bakowsky U.
中科院分区:
--
文献类型:
--
作者:
Vidawati S;Sitterberg J;Rothe U;Bakowsky U.

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采用Langmuir薄膜平衡、椭偏和原子力显微镜(AFM)的组合方法研究了天然不对称和对称的bolaamphi亲性物质(古细菌四醚脂)的单分子组织。将单层薄膜转移到硅片衬底上。将脂类转移到几种硅片衬底(疏水、亲水性和氨基硅化)上后,通过椭偏仪和原子力显微镜进一步研究了单层膜,以确定亲水两亲分子在水-空气界面上的取向是否不同,包括“马蹄形”和“直立”构型。由椭偏法测定的单层膜的厚度代表了这些畴的组合平均值。利用原子力显微镜(AFM)对表面形貌进行了评估,结果表明,在所有薄膜中,畴都排列在小域或最均匀的组织中,这取决于晶圆硅衬底的性质。在所有薄膜中,疏水转移的脂类在底物上表现出最均匀的组织结构。在转移到亲水性和氨基硅化表面后,脂质在底物上排列成小岛屿。
The monomolecular organisation of the natural asymmetric and symmetric bolaamphiphilic (archaebacterial tetraether lipids) was studied using a combination of Langmuir film balance, ellipsometry, and atomic force microscopy (AFM). Monolayer films were transferred onto silicon wafer substrates. After transfer of the lipids onto several kinds of silicon wafer substrates (hydrophobic, hydrophilic, and amino-silanised), the monolayer films were further investigated by ellipsometry and AFM in order to determined whether bolaamphiphiles are oriented differently at the water–air interface, including “horseshoe” and “upright” configurations. The thickness of the monolayer film, which was determined by ellipsometry, represents a combined mean value of the domains. The surface morphology, which was evaluated by AFM showed that in all films, the domains were arranged in small domains or the most homogeneous organisation, depending on the properties of the wafer silicon substrates. From all films, the hydrophobically transferred lipids showed the most homogeneous organisation on the substrates. After transfer onto hydrophilic and amino-silanised surfaces, the lipids were arranged in small islands on the substrates.
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