Comparative glycomics of leukocyte glycosaminoglycans.
Comparative glycomics of leukocyte glycosaminoglycans.
复制标题
DOI：
10.1111/febs.12231
复制
发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Zaia J
中科院分区：
文献类型：
--
作者：
Shao C;Shi X;White M;Huang Y;Hartshorn K;Zaia J
糖胺聚糖（GAG）的二糖和寡糖含量以组织特异性方式变化很大。尽管如此，有共同的结构特征，如硫酸乙酰肝素（HS）链上存在高度硫酸化的非还原性末端结构域。不太清楚的是GAG在特定细胞类型上的表达模式。已知白细胞主要表达硫酸软骨素（CS）型GAG。然而，关于GAG链的性质和结构、其在正常受试者中的变异范围以及与疾病状况相关的结构变化，知之甚少。我们从四个人供体中分离外周血白细胞群并提取GAG。我们确定了相对和绝对的二糖丰度HS和CS GAG分类使用尺寸排阻色谱-质谱法（SEC-MS）。我们发现，所有的白细胞表达HS链的硫酸化水平更类似于肝素比器官来源的HS。HS表达水平遵循T细胞、B细胞>单核细胞、NK细胞>多形核白细胞（PMNs）的趋势。此外，CS丰度大大高于总HS，但在白细胞类型的具体方式有很大的不同。骨髓系细胞（PMN，单核细胞）的CS水平高于淋巴样细胞（B，T，NK细胞）。该信息确定了正常白细胞上表达的GAG结构的范围，并且是随后调查疾病状况所必需的。
Glycosaminoglycans (GAGs) vary widely in disaccharide and oligosaccharide content in a tissue-specific manner. Nonetheless, there are common structural features, such as the presence of highly sulfated non-reducing end domains on heparan sulfate (HS) chains. Less clear are the patterns of expression of GAGs on specific cell types. Leukocytes are known to express GAGs primarily of the chondroitin sulfate (CS) type. Little is known, however, regarding the properties and structures of the GAG chains, their ranges of variability among normal subjects, and changes in structure associated with disease conditions. We isolated peripheral blood leukocyte populations from four human donors and extracted GAGs. We determined the relative and absolute disaccharides abundances for HS and CS GAG classed using size exclusion chromatography-mass spectrometry (SEC-MS). We found that all leukocytes express HS chains with level of sulfation more similar to heparin than to organ-derived HS. The levels of HS expression follows the trend T Cells, B cells>monocytes, NK cells>polymorphonuclear leukocytes (PMNs). In addition, CS abundances were considerably higher than total HS but varied considerably in a leukocyte cell type specific manner. Levels of CS were higher for myeloid lineage cells (PMNs, monocytes) than for lymphoid cells (B, T, NK cells). This information establishes the ranges of GAG structures expressed on normal leukocytes and necessary for subsequent inquiry into disease conditions.
登录
查看更多内容
影响因子：
3
作者：
Fadnes, Bodil;Husebekk, Anne;Svineng, Gunbjorg;Rekdal, Oystein;Yanagishita, Masaki;Kolset, Svein O.;Uhlin-Hansen, Lars
通讯作者：
Uhlin-Hansen, Lars
影响因子：
4.8
作者：
Maccarana, M;Olander, B;Malmström, A
通讯作者：
Malmström, A
影响因子：
4.8
作者：
Maccarana, M;Sakura, Y;Lindahl, U
通讯作者：
Lindahl, U
影响因子：
5.4
作者：
Jones, KS;Petrow-Sadowski, C;Ruscetti, FW
通讯作者：
Ruscetti, FW
影响因子：
7.4
作者：
Desaire, H;Sirich, TL;Leary, JA
通讯作者：
Leary, JA