The mechanical properties of individual, electrospun fibrinogen fibers.
The mechanical properties of individual, electrospun fibrinogen fibers.
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DOI：
10.1016/j.biomaterials.2008.11.006
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发表时间：
2009-02
期刊：
影响因子：
14
通讯作者：
Guthold, Martin
中科院分区：
文献类型：
--
作者：
Carlisle, Christine R.;Coulais, Corentin;Namboothiry, Manoj;Carroll, David L.;Hantgan, Roy R.;Guthold, Martin
我们使用原子力显微镜 (AFM)/荧光显微镜相结合的技术来研究水缓冲液中单个电纺纤维蛋白原纤维的机械性能。纤维（平均直径 208 nm）悬浮在条纹透明基底上 12 μm 宽的凹槽上。位于样品上方的 AFM 用于横向拉伸纤维并测量所施加的力。位于样品下方的荧光显微镜用于观察拉伸过程。纤维在断裂前可拉伸至原长度的2.3倍；断裂应力为 22 × 106 Pa。我们收集了增量应力-应变曲线来确定这些纤维的粘弹性行为。总拉伸模量为 17.5 × 106 Pa，松弛弹性模量为 7.2 × 106 Pa。当保持恒定应变时，电纺纤维蛋白原纤维表现出分别为 3 秒和 55 秒的快应力松弛时间和慢应力松弛时间。我们的纤维由通常使用的 90% 1,1,1,3,3,3-六氟-2-丙醇 (90-HFP) 静电纺丝溶液纺成，并重新悬浮在水性缓冲液中。圆二色光谱表明，90-HFP 中纤维蛋白原的 α-螺旋含量比水溶液中高约 70%。需要这些数据来了解电纺纤维蛋白原结构的机械行为。我们的技术也适用于研究其他纳米级纤维。
We used a combined atomic force microscopic (AFM)/fluorescence microscopic technique to study the mechanical properties of individual, electrospun fibrinogen fibers in aqueous buffer. Fibers (average diameter 208 nm) were suspended over 12 μm-wide grooves in a striated, transparent substrate. The AFM, situated above the sample, was used to laterally stretch the fibers and to measure the applied force. The fluorescence microscope, situated below the sample, was used to visualize the stretching process. The fibers could be stretched to 2.3 times their original length before breaking; the breaking stress was 22 × 106 Pa. We collected incremental stress–strain curves to determine the viscoelastic behavior of these fibers. The total stretch modulus was 17.5 × 106 Pa and the relaxed elastic modulus was 7.2 × 106 Pa. When held at constant strain, electrospun fibrinogen fibers showed a fast and slow stress relaxation time of 3 and 55 s. Our fibers were spun from the typically used 90% 1,1,1,3,3,3-hexafluoro-2-propanol (90-HFP) electrospinning solution and re-suspended in aqueous buffer. Circular dichroism spectra indicate that α-helical content of fibrinogen is ~70% higher in 90-HFP than in aqueous solution. These data are needed to understand the mechanical behavior of electrospun fibrinogen structures. Our technique is also applicable to study other nanoscopic fibers.
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发表时间：
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影响因子：
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发表时间：
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影响因子：
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