Spatiotemporal characterization of microdamage accumulation in rat ulnae in response to uniaxial compressive fatigue loading.
Spatiotemporal characterization of microdamage accumulation in rat ulnae in response to uniaxial compressive fatigue loading.
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大鼠尺骨微损伤累积响应单轴压缩疲劳载荷的时空特征。
DOI：
10.1016/j.bone.2018.01.011
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发表时间：
2018-03
期刊：
影响因子：
4.1
通讯作者：
Jing D
中科院分区：
文献类型：
--
作者：
Zhang X;Liu X;Yan Z;Cai J;Kang F;Shan S;Wang P;Zhai M;Guo XE;Luo E;Jing D
重复疲劳载荷会引起骨基质中微损伤的积累，从而导致机械性能受损并增加骨折敏感性。然而，疲劳负载骨骼中微损伤累积的空间分布和时变过程，尤其是已知会引发骨重塑的线性微裂纹，仍不完全清楚。本研究研究了单轴压缩疲劳载荷作用下大鼠尺骨微裂纹形态和分布的时变过程。 34月龄雄性Sprague-Dawley大鼠的右前肢以0.67 Hz、归一化峰值力0.055 N/g体重进行一轮循环斜坡加载6000个循环，对侧左尺骨不作为对照样本加载。疲劳负荷后第 3、5 和 7 天，将 10 只大鼠随机安乐死。我们通过基于基本品红染色的二维组织形态测量和使用对比增强微型计算机断层扫描 (MicroCT) 和沉淀 BaSO4 染色的三维定量结果表明，疲劳加载后第 5 天线性微裂纹的积累（线性微裂纹数量的增加）显着高于第 3 天和第 7 天。我们的组织学和组织形态测量结果表明，疲劳加载后第 3、5 和 7 天拉伸皮质中的线性微裂纹密度 (Cr.Dn) 显着高于压缩侧，而第 3 天拉伸皮质中的线性微裂纹长度 (Cr.Le) 显着低于压缩侧皮质。我们的研究结果表明，微裂纹积累在轴向压缩疲劳载荷后 3、5 和 7 天表现出非线性时变过程（在第 5 天可观察到峰值 Cr.Dn）。我们的研究结果还揭示了具有拉伸和压缩应变的大鼠尺骨中微裂纹密度和形态的明显分布，其特征是拉伸皮质中积累了更多的微裂纹，而压缩皮质中显示出更长的裂纹。
Repetitive fatigue loading can induce microdamage accumulation in bone matrix, which results in impaired mechanical properties and increased fracture susceptibility. However, the spatial distribution and time-variant process of microdamage accumulation in fatigue-loaded skeleton, especially for linear microcracks which are known to initiate bone remodeling, remain not fully understood. In this study, the time-varying process of the morphology and distribution of microcracks in rat ulnae subjected to uniaxial compressive fatigue loading was investigated. Right forelimbs of thirty four-month-old male Sprague-Dawley rats were subjected to one bout of cyclic ramp loading with 0.67 Hz at a normalized peak force of 0.055 N/g body weight for 6000 cycles, and the contralateral left ulnae were not loaded as the control samples. Ten rats were randomly euthanized on Days 3, 5, and 7 post fatigue loading. Our findings via two-dimensional histomorphometric measurements based on basic fuchsin staining and three-dimensional quantifications using contrast-enhanced micro-computed tomography (MicroCT) with precipitated BaSO4 staining demonstrated that the accumulation of linear microcracks (increase in the amount of linear microcracks) on Day 5 was significantly higher than that on Day 3 and Day 7 post fatigue loading. Our histological and histomorphometric results revealed that linear microcrack density (Cr.Dn) in the tensile cortex at Days 3, 5 and 7 post fatigue loading was significantly higher than that in the compressive side, whereas linear microcrack length (Cr.Le) in the tensile cortex at Day 3 was significantly lower than that in the compressive cortex. Our findings revealed that microcrack accumulation exhibited a non-linear time-varying process at 3, 5 and 7 days post axial compressive fatigue loading (with observable peak Cr.Dn at Day 5). Our findings also revealed distinct distribution of microcrack density and morphology in rat ulnae with tensile and compressive strains, as characterized by more microcracks accumulated in tensile cortices, and longer cracks shown in compressive cortices.
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影响因子：
2.4
作者：
Xiang-Shuo Wang;Daniel B Masse;H. Leng;Kevin P Hess;R. Ross;R. Roeder;G. Niebur
通讯作者：
Xiang-Shuo Wang;Daniel B Masse;H. Leng;Kevin P Hess;R. Ross;R. Roeder;G. Niebur
影响因子：
17.3
作者：
O. J. Vaughan-Jackson;M B Devas
通讯作者：
O. J. Vaughan-Jackson;M B Devas
影响因子：
6.2
作者：
B. Uthgenannt;Michael H Kramer;Joyce A Hwu;B. Wopenka;Matthew J. Silva
通讯作者：
B. Uthgenannt;Michael H Kramer;Joyce A Hwu;B. Wopenka;Matthew J. Silva
DOI：
10.2739/kurumemedj.30.143
发表时间：
1983
期刊：
The Kurume Medical Journal
影响因子：
--
作者：
H. Inoue;S. Tahira
通讯作者：
H. Inoue;S. Tahira
影响因子：
2.7
作者：
Nor Hazla Mohamed Haflah;Noreen Fazlina Mat Nor;S. Abdullah;J. Sapuan
通讯作者：
Nor Hazla Mohamed Haflah;Noreen Fazlina Mat Nor;S. Abdullah;J. Sapuan