Silencing the HaHR3 gene by transgenic plant-mediated RNAi to disrupt Helicoverpa armigera development.
Silencing the HaHR3 gene by transgenic plant-mediated RNAi to disrupt Helicoverpa armigera development.
复制标题
DOI：
10.7150/ijbs.5929
复制
发表时间：
2013
影响因子：
9.2
通讯作者：
Qiu D
中科院分区：
文献类型：
--
作者：
Xiong Y;Zeng H;Zhang Y;Xu D;Qiu D
外源双链RNA （dsRNA）引起的RNA干扰（RNAi）已发展成为功能基因组学中的一项重要技术，目前已被广泛用于下调害虫关键生理相关基因以控制害虫。选择棉铃虫（Helicoverpa armigera）脱毛调控转录因子基因HaHR3作为靶基因。将覆盖HaHR3编码序列（CDS）的4个不同片段克隆到大肠杆菌载体L4440中表达dsRNAs。然后将最有效的沉默片段克隆到植物过表达载体中，在转基因烟草中表达发夹RNA （hpRNA）。以大肠杆菌或转基因植物为食，棉铃虫幼虫HaHR3 mRNA和蛋白水平显著降低，导致发育畸形和幼虫死亡。结果表明，重组细菌和转基因植物都可以诱导HaHR3沉默，从而破坏棉铃虫的发育，转基因植物介导的RNAi正在成为一种强有力的害虫防治手段。
RNA interference (RNAi) caused by exogenous double-stranded RNA (dsRNA) has developed into a powerful technique in functional genomics, and to date it is widely used to down-regulate crucial physiology-related genes to control pest insects. A molt-regulating transcription factor gene, HaHR3, of cotton bollworm (Helicoverpa armigera) was selected as the target gene. Four different fragments covering the coding sequence (CDS) of HaHR3 were cloned into vector L4440 to express dsRNAs in Escherichia coli. The most effective silencing fragment was then cloned into a plant over-expression vector to express a hairpin RNA (hpRNA) in transgenic tobacco (Nicotiana tabacum). When H. armigera larvae were fed the E. coli or transgenic plants, the HaHR3 mRNA and protein levels dramatically decreased, resulting developmental deformity and larval lethality. The results demonstrate that both recombinant bacteria and transgenic plants could induce HaHR3 silence to disrupt H. armigera development, transgenic plant-mediated RNAi is emerging as a powerful approach for controlling insect pests.
登录
查看更多内容
影响因子：
14.9
作者：
Ding, Y;Chan, CY;Lawrence, CE
通讯作者：
Lawrence, CE
影响因子：
2.6
作者：
Cruz, Josefa;Martin, David;Belles, Xavier
通讯作者：
Belles, Xavier
DOI：
10.1073/pnas.94.22.12024
发表时间：
1997-10-28
影响因子：
11.1
作者：
Carney, GE;Wade, AA;Bender, M
通讯作者：
Bender, M
影响因子：
12.3
作者：
通讯作者：
--
影响因子：
3.7
作者：
Kumar P;Pandit SS;Baldwin IT
通讯作者：
Baldwin IT