The heat shock protein Ssa2p is required for import of fructose-1, 6-bisphosphatase into Vid vesicles.
The heat shock protein Ssa2p is required for import of fructose-1, 6-bisphosphatase into Vid vesicles.
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将果糖-1, 6-双磷酸酶导入 Vid 囊泡需要热休克蛋白 Ssa2p。
DOI：
10.1083/jcb.150.1.65
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发表时间：
2000-07-10
期刊：
影响因子：
--
通讯作者：
Chiang HL
中科院分区：
文献类型：
--
作者：
Brown CR;McCann JA;Chiang HL
当酿酒酵母从低糖转变为高糖时，果糖-1，6-二磷酸酶(FBPase)被定位在液泡中进行降解。然而，在液泡导入之前，FBPase被隔离在一种新型的囊泡内，即液泡导入和降解(VID)囊泡。在这里，我们重建了FBPase的导入到分离的VID囊泡中。FBPase在VID囊泡中的隔离需要ATP和胞浆，但如果胞浆中的ATP结合蛋白被耗尽，则FBPase被抑制。热休克蛋白Ssa2p被鉴定为参与FBPase输入的ATP结合蛋白之一。Δssa2菌株体内FBPase降解率显著低于Δssa1、Δssa3或Δssa4菌株。同样，Δssa2株的体外进口受到损害，但其他Δssa株的体外进口没有受到影响。胞浆被鉴定为Δssa2缺陷的部位；Δssa2胞浆不能刺激FbPase导入到可导入的VID囊泡中，但野生型胞浆支持FbPase导入到Δssa2囊泡中。在ΔsSA2胞浆存在的情况下，加入纯化的重组SSA2p可刺激FBPase导入ΔsSA2 VID囊泡。因此，Ssa2p以及其他未定义的胞浆蛋白是FBPase进入囊泡所必需的。
Fructose-1,6-bisphosphatase (FBPase) is targeted to the vacuole for degradation when Saccharomyces cerevisiae are shifted from low to high glucose. Before vacuolar import, however, FBPase is sequestered inside a novel type of vesicle, the vacuole import and degradation (Vid) vesicles. Here, we reconstitute import of FBPase into isolated Vid vesicles. FBPase sequestration into Vid vesicles required ATP and cytosol, but was inhibited if ATP binding proteins were depleted from the cytosol. The heat shock protein Ssa2p was identified as one of the ATP binding proteins involved in FBPase import. A Δssa2 strain exhibited a significant decrease in the rate of FBPase degradation in vivo as compared with Δssa1, Δssa3, or Δssa4 strains. Likewise, in vitro import was impaired for the Δssa2 strain, but not for the other Δssa strains. The cytosol was identified as the site of the Δssa2 defect; Δssa2 cytosol did not stimulate FBPase import into import competent Vid vesicles, but wild-type cytosol supported FBPase import into competent Δssa2 vesicles. The addition of purified recombinant Ssa2p stimulated FBPase import into Δssa2 Vid vesicles, providing Δssa2 cytosol was present. Thus, Ssa2p, as well as other undefined cytosolic proteins are required for the import of FBPase into vesicles.
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影响因子：
7.8
作者：
Baba, M;Osumi, M;Scott, SV;Klionsky, DJ;Ohsumi, Y
通讯作者：
Ohsumi, Y
酵母突变体在细胞质中的分离和表征与液泡蛋白靶向途径的分离。
影响因子：
7.8
作者：
HARDING, TM;MORANO, KA;SCOTT, SV;KLIONSKY, DJ
通讯作者：
KLIONSKY, DJ
DOI：
10.1083/jcb.137.4.825
发表时间：
1997-05-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Agarraberes FA;Terlecky SR;Dice JF
通讯作者：
Dice JF
影响因子：
56.9
作者：
CHIANG, HL;TERLECKY, SR;DICE, JF
通讯作者：
DICE, JF
影响因子：
4.8
作者：
Chiang, HL;Schekman, R;Hamamoto, S
通讯作者：
Hamamoto, S