Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway.
Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway.
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酵母突变体在细胞质中的分离和表征与液泡蛋白靶向途径的分离。
DOI：
10.1083/jcb.131.3.591
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发表时间：
1995-11
影响因子：
7.8
通讯作者：
KLIONSKY, DJ
中科院分区：
文献类型：
--
作者：
HARDING, TM;MORANO, KA;SCOTT, SV;KLIONSKY, DJ
在酿酒酵母（Saccharomyces cerevisiae）中，液泡蛋白质氨肽酶I（API）定位于液泡，且不依赖于分泌途径。这种蛋白质所使用的替代靶向机制尚未被阐明。API是作为一种61 - kD的可溶性胞质前体合成的。在被运输到液泡后，其氨基末端前肽会被蛋白酶B（PrB）切除，从而产生成熟的50 - kD水解酶。我们在一次突变体筛选中利用这种依赖运输的成熟事件来鉴定其产物参与API靶向的基因。利用针对API前肽的抗血清，我们分离出了积累API前体的突变体。这些被命名为cvt的突变体可分为八个互补群，其中五个互补群定义了新的基因。这五个互补群在API的成熟过程中表现出特定的缺陷，但对通过分泌途径的液泡蛋白质靶向没有显著影响。定位研究表明，在所有五个互补群中，API前体都在液泡外积累，这表明它们在API靶向和/或易位过程中受阻。对这些基因产物的进一步分析将提供有关参与这种液泡蛋白质定位替代机制的亚细胞组分的信息。
In Saccharomyces cerevisiae the vacuolar protein aminopeptidase I (API) is localized to the vacuole independent of the secretory pathway. The alternate targeting mechanism used by this protein has not been characterized. API is synthesized as a 61-kD soluble cytosolic precursor. Upon delivery to the vacuole, the amino-terminal propeptide is removed by proteinase B (PrB) to yield the mature 50-kD hydrolase. We exploited this delivery-dependent maturation event in a mutant screen to identify genes whose products are involved in API targeting. Using antiserum to the API propeptide, we isolated mutants that accumulate precursor API. These mutants, designated cvt, fall into eight complementation groups, five of which define novel genes. These five complementation groups exhibit a specific defect in maturation of API, but do not have a significant effect on vacuolar protein targeting through the secretory pathway. Localization studies show that precursor API accumulates outside of the vacuole in all five groups, indicating that they are blocked in API targeting and/or translocation. Future analysis of these gene products will provide information about the subcellular components involved in this alternate mechanism of vacuolar protein localization.
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影响因子：
7.8
作者：
EMR, SD;VASSAROTTI, A;DOUGLAS, MG
通讯作者：
DOUGLAS, MG
影响因子：
64.5
作者：
JENNESS, DD;BURKHOLDER, AC;HARTWELL, LH
通讯作者：
HARTWELL, LH
DOI：
10.1083/jcb.108.4.1271
发表时间：
1989-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bankaitis VA;Malehorn DE;Emr SD;Greene R
通讯作者：
Greene R
酵母自噬过程的超微结构分析：自噬体的检测及其表征。
影响因子：
7.8
作者：
Baba, M;Takeshige, K;Baba, N;Ohsumi, Y
通讯作者：
Ohsumi, Y
DOI：
10.1073/pnas.83.23.9075
发表时间：
1986-12-01
影响因子：
11.1
作者：
BANKAITIS, VA;JOHNSON, LM;EMR, SD
通讯作者：
EMR, SD