The Paracoccus denitrificans NarK-like nitrate and nitrite transporters-probing nitrate uptake and nitrate/nitrite exchange mechanisms.
The Paracoccus denitrificans NarK-like nitrate and nitrite transporters-probing nitrate uptake and nitrate/nitrite exchange mechanisms.
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DOI：
10.1111/mmi.13546
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发表时间：
2017-01
影响因子：
3.6
通讯作者：
Ferguson SJ
中科院分区：
文献类型：
--
作者：
Goddard AD;Bali S;Mavridou DA;Luque-Almagro VM;Gates AJ;Dolores Roldán M;Newstead S;Richardson DJ;Ferguson SJ
硝酸盐和亚硝酸盐跨生物膜的转运通常由蛋白质转运蛋白促进，这些蛋白质转运蛋白是主要的易化剂超家族的成员。副球菌包含一个不寻常的安排，其中两个这些转运蛋白，NarK 1和NarK 2，融合成一个单一的蛋白质，NarK，提供硝酸盐的呼吸硝酸还原酶和转移产品，亚硝酸盐，到周质。我们的互补研究，使用的突变体缺乏硝酸盐/质子同向转运体NasA从同化硝酸还原酶途径，支持NarK 1作为硝酸盐/质子同向转运体，而NarK 2是硝酸盐/亚硝酸盐反向转运体。通过相同的实验系统，我们发现大肠杆菌NarK和NarU可以补充P. acetificans中narK和nasA的缺失，这表明，虽然这些蛋白质最有可能是硝酸盐/亚硝酸盐反向转运蛋白，但它们也可以在硝酸盐的净摄取中起作用。最后，我们认为，一级序列分析和结构建模并不容易解释为什么NasA，NarK 1和NarK 2，以及其他转运蛋白从这个蛋白质家族，有这样不同的功能，从净硝酸盐摄取硝酸盐/亚硝酸盐交换。
Nitrate and nitrite transport across biological membranes is often facilitated by protein transporters that are members of the major facilitator superfamily. Paracoccus denitrificans contains an unusual arrangement whereby two of these transporters, NarK1 and NarK2, are fused into a single protein, NarK, which delivers nitrate to the respiratory nitrate reductase and transfers the product, nitrite, to the periplasm. Our complementation studies, using a mutant lacking the nitrate/proton symporter NasA from the assimilatory nitrate reductase pathway, support that NarK1 functions as a nitrate/proton symporter while NarK2 is a nitrate/nitrite antiporter. Through the same experimental system, we find that Escherichia coli NarK and NarU can complement deletions in both narK and nasA in P. denitrificans, suggesting that, while these proteins are most likely nitrate/nitrite antiporters, they can also act in the net uptake of nitrate. Finally, we argue that primary sequence analysis and structural modelling do not readily explain why NasA, NarK1 and NarK2, as well as other transporters from this protein family, have such different functions, ranging from net nitrate uptake to nitrate/nitrite exchange.
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DOI：
10.1038/nsb821
发表时间：
2002-08-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Hirai, T;Heymann, JAW;Subramaniam, S
通讯作者：
Subramaniam, S
影响因子：
16.6
作者：
Fukuda, Masahiro;Takeda, Hironori;Kato, Hideaki E.;Doki, Shintaro;Ito, Koichi;Maturana, Andres D.;Ishitani, Ryuichiro;Nureki, Osamu
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DOI：
10.1073/pnas.1210793109
发表时间：
2012-11-06
影响因子：
11.1
作者：
Lu, Wei;Schwarzer, Nikola J.;Einsle, Oliver
通讯作者：
Einsle, Oliver
DOI：
10.1016/0005-2728(83)90049-x
发表时间：
1983-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
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作者：
BOOGERD, FC;VANVERSEVELD, HW;STOUTHAMER, AH
通讯作者：
STOUTHAMER, AH
DOI：
10.1042/bj20150880
发表时间：
2016-02-01
期刊：
The Biochemical journal
影响因子：
--
作者：
Cabrera JJ;Salas A;Torres MJ;Bedmar EJ;Richardson DJ;Gates AJ;Delgado MJ
通讯作者：
Delgado MJ