Dynamics of Viral and Proviral Loads of FelineImmunodeficiency Virus within the Feline Central Nervous System duringthe Acute Phase following IntravenousInfection
Dynamics of Viral and Proviral Loads of FelineImmunodeficiency Virus within the Feline Central Nervous System duringthe Acute Phase following IntravenousInfection
复制标题
静脉感染后急性期猫中枢神经系统内猫免疫缺陷病毒病毒和原病毒载量的动态
作者：
G. Ryan;D. Klein;E. Knapp;M. Hosie;T. Grimes;M. F. Mabruk;O. Jarrett;J. Callanan
摘要人类免疫缺陷病毒 1 的动物模型，例如猫免疫缺陷病毒 (FIV)，为剖析病毒与中枢神经系统 (CNS) 早期相互作用的机制提供了机会。本研究的目的是评估猫静脉接种 FIVGL8 后前 23 周内中枢神经系统、脑脊液 (CSF)、眼液和血浆中的病毒载量。还测定了外周血单核细胞 (PBMC) 和脑组织中的前病毒载量。在感染的急性阶段，病毒进入大多数动物的大脑。病毒分布最初是随机的，随着感染的进展，大脑受累更加分散。脑脊液中的病毒预示着脑实质感染。虽然血液中病毒产生的峰值与大脑内的增殖同时发生，但脑组织中似乎仍在继续产生更持续的病毒。相比之下，在 PBMC 负荷增强的情况下，大脑中的前病毒负荷下降至不可检测的水平。这项研究的最终观察结果是，大脑或眼组织区域的病毒载量与组织病理学的存在之间没有直接相关性。
ABSTRACT Animal models of human immunodeficiency virus 1, such as feline immunodeficiency virus (FIV), provide the opportunities to dissect the mechanisms of early interactions of the virus with the central nervous system (CNS). The aims of the present study were to evaluate viral loads within CNS, cerebrospinal fluid (CSF), ocular fluid, and the plasma of cats in the first 23 weeks after intravenous inoculation with FIVGL8. Proviral loads were also determined within peripheral blood mononuclear cells (PBMCs) and brain tissue. In this acute phase of infection, virus entered the brain in the majority of animals. Virus distribution was initially in a random fashion, with more diffuse brain involvement as infection progressed. Virus in the CSF was predictive of brain parenchymal infection. While the peak of virus production in blood coincided with proliferation within brain, more sustained production appeared to continue in brain tissue. In contrast, proviral loads in the brain decreased to undetectable levels in the presence of a strengthening PBMC load. A final observation in this study was that there was no direct correlation between viral loads in regions of brain or ocular tissue and the presence of histopathology.
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人类免疫缺陷病毒1型宿主：根除病毒的主要障碍。
DOI：
10.1086/338256
发表时间：
2002
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.
影响因子：
--
作者：
Pomerantz,RogerJ
通讯作者：
Pomerantz,RogerJ
实验性猫免疫缺陷病毒感染引起的艾滋病相关脑病。
DOI：
--
发表时间：
1993
期刊：
Journal of acquired immune deficiency syndromes
影响因子：
--
作者：
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通讯作者：
Lafrado,L
HIV-1 脑炎及其相关痴呆动物模型系统的开发。
DOI：
10.3109/13550289509114019
发表时间：
1995
期刊：
Journal of neurovirology.
影响因子：
--
作者：
Persidsky,Y;Nottet,HS;Sasseville,VG;Epstein,LG;Gendelman,HE
通讯作者：
Gendelman,HE
影响因子：
1
作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen
通讯作者：
J. Yamamoto;E. Sparger;E. Ho;P. Andersen;T. O'connor;C. P. Mandell;L. Lowenstine;R. Munn;N. Pedersen
影响因子：
1.5
作者：
Leutenegger, CM;Higgins, J;North, TW
通讯作者：
North, TW