A Mutant at Position 87 of the GroEL Chaperonin Is Affected in Protein Binding and ATP Hydrolysis (*)
A Mutant at Position 87 of the GroEL Chaperonin Is Affected in Protein Binding and ATP Hydrolysis (*)
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GroEL Chaperonin 87 位突变体影响蛋白质结合和 ATP 水解 (*)
DOI：
10.1074/jbc.270.23.13956
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
P. Goloubinoff
中科院分区：
文献类型：
--
作者：
C. Weiss;P. Goloubinoff
将大肠杆菌伴侣蛋白GroEL的87位高度保守的天冬氨酸残基突变为谷氨酸。当在E.在噬菌体形态发生缺陷的coligroEL突变株中，质粒编码的GroEL突变体D87 E恢复了T4噬菌体形态发生。然而，它没有重新激活来自费氏弧菌的重组荧光素酶操纵子的转录。在体外，GroEL突变体D87 E被发现在结合非天然蛋白质和水解ATP的能力受损，导致尿素变性核酮糖-1，5-二磷酸羧化酶/加氧酶的复性效率较低。突变体寡聚体D87 E GroEL 14能够与野生型GroEL 14一样有效地结合GroES 7。位于GroEL的赤道结构域中的位置87处的保守天冬氨酸残基（Braig，K.，Otwinowski，Z.，Hegde河，布瓦韦特，D。C.的方法，Joachimiak，A.，Horwich，A. L.，和Sigler，P. B.（1994）Nature 371，578-586）因此推断对非天然蛋白质与GroEL 14核心伴侣蛋白的结合和对ATP水解具有双重作用。
The highly conserved aspartic acid residue at position 87 of the Escherichia coli chaperonin GroEL was mutated to glutamic acid. When expressed in an E. coligroEL mutant strain deficient for phage morphogenesis, plasmid-encoded GroEL mutant D87E restored T4 phage morphogenesis. It did not, however, reactivate the transcription of a recombinant luciferase operon from Vibrio fischeri. In vitro, GroEL mutant D87E was found to be impaired in the ability to bind nonnative proteins and to hydrolyze ATP, resulting in less efficient refolding of urea-denatured ribulose-1,5-bisphosphate carboxylase/oxygenase. Mutant oligomer D87E GroEL14 was able to bind GroES7 as efficiently as wild-type GroEL14. The conserved aspartic acid residue at position 87 located in the equatorial domain of GroEL (Braig, K., Otwinowski, Z., Hegde, R., Boisvert, D. C., Joachimiak, A., Horwich, A. L., and Sigler, P. B.(1994) Nature 371, 578-586) is thus inferred to have a dual effect on the binding of nonnative proteins to the GroEL14 core chaperonin and on ATP hydrolysis.
DOI：
10.1016/s0021-9258(18)98800-9
发表时间：
1991-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. A. Mendoza;E. Rogers;G. Lorimer;Paul M. Horowitz
通讯作者：
J. A. Mendoza;E. Rogers;G. Lorimer;Paul M. Horowitz
大肠杆菌 groES 形态发生蛋白的纯化和性质。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Chandrasekhar,GN;Tilly,K;Woolford,C;Hendrix,R;Georgopoulos,C
通讯作者：
Georgopoulos,C
DOI：
10.1073/pnas.78.3.1629
发表时间：
1981-03
影响因子：
11.1
作者：
K. Tilly;H. Murialdo;C. Georgopoulos
通讯作者：
K. Tilly;H. Murialdo;C. Georgopoulos