Spatial patterning of excitatory and inhibitory neuropil territories during spinal circuit development.
Spatial patterning of excitatory and inhibitory neuropil territories during spinal circuit development.
复制标题
DOI：
10.1002/cne.24152
复制
发表时间：
2017-05-01
期刊：
影响因子：
--
通讯作者：
Dallman JE
中科院分区：
文献类型：
--
作者：
Yan Q;Zhai L;Zhang B;Dallman JE
为了产生有节奏的运动行为，单个神经元和神经回路都需要在触发动作电位的兴奋性输入和促进稳定的静息电位的抑制性输入（E/I平衡）之间保持平衡。以前的研究集中在单个神经元，并已表明，在一个短的空间尺度上，兴奋性和抑制性（E/I）突触往往形成结构化的领土与抑制性输入丰富的细胞体和近端树突和兴奋性输入远端树突。然而，系统级的E/I模式，在空间尺度大于单个神经元，在很大程度上是未知的。我们使用PSD-95和gephyrin突触后支架蛋白作为兴奋性和抑制性突触的代理，分别进行免疫染色，以量化斑马鱼脊髓中E/I突触的数量和分布，在胚胎阶段和幼虫阶段。在胚胎期，我们发现PSD-95点的数量超过桥蛋白点，随着桥蛋白点的数量增加，以匹配PSD-95点在幼虫期。在这两个阶段，PSD-95斑点富集在对应于远端树突的最外侧神经毡中，而桥蛋白斑点富集在神经元胞体和内侧神经毡中。值得注意的是，类似于突触点，神经元的过程也表现出内侧-外侧的领土在两个发展阶段与丰富的兴奋性（兴奋性）过程横向和甘氨酸（抑制性）过程中间。这种神经元兴奋-抑制结构的建立在很大程度上先于初级运动神经元的树突状分支，这表明结构化的神经元可以在细胞水平上为E/I平衡的发展提供框架。
To generate rhythmic motor behaviors, both single neurons and neural circuits require a balance between excitatory inputs that trigger action potentials and inhibitory inputs that promote a stable resting potential (E/I balance). Previous studies have focused on individual neurons and have shown that, over a short spatial scale, excitatory and inhibitory (E/I) synapses tend to form structured territories with inhibitory inputs enriched on cell bodies and proximal dendrites and excitatory inputs on distal dendrites. However, systems-level E/I patterns, at spatial scales larger than single neurons, are largely uncharted. We used immunostaining for PSD-95 and gephyrin postsynaptic scaffolding proteins as proxies for excitatory and inhibitory synapses, respectively, to quantify the numbers and map the distributions of E/I synapses in zebrafish spinal cord at both an embryonic stage and a larval stage. At the embryonic stage, we found that PSD-95 puncta out-number gephyrin puncta, with the number of gephyrin puncta increasing to match that of PSD-95 puncta at the larval stage. At both stages, PSD-95 puncta are enriched in the most lateral neuropil corresponding to distal dendrites while gephyrin puncta are enriched on neuronal somata and in the medial neuropil. Significantly, similar to synaptic puncta, neuronal processes also exhibit medial-lateral territories at both developmental stages with enrichment of glutamatergic (excitatory) processes laterally and glycinergic (inhibitory) processes medially. This establishment of neuropil excitatory-inhibitory structure largely precedes dendritic arborization of primary motor neurons, suggesting that the structured neuropil could provide a framework for the development of E/I balance at the cellular level.
登录
查看更多内容
影响因子：
5.3
作者：
Flores CE;Méndez P
通讯作者：
Méndez P
影响因子：
30.8
作者：
Abrams AJ;Hufnagel RB;Rebelo A;Zanna C;Patel N;Gonzalez MA;Campeanu IJ;Griffin LB;Groenewald S;Strickland AV;Tao F;Speziani F;Abreu L;Schüle R;Caporali L;La Morgia C;Maresca A;Liguori R;Lodi R;Ahmed ZM;Sund KL;Wang X;Krueger LA;Peng Y;Prada CE;Prows CA;Schorry EK;Antonellis A;Zimmerman HH;Abdul-Rahman OA;Yang Y;Downes SM;Prince J;Fontanesi F;Barrientos A;Németh AH;Carelli V;Huang T;Zuchner S;Dallman JE
通讯作者：
Dallman JE
影响因子：
4.5
作者：
Brustein, E;Marandi, N;Konnerth, A
通讯作者：
Konnerth, A
影响因子：
5.3
作者：
Fong, DK;Rao, A;Craig, AM
通讯作者：
Craig, AM
影响因子：
3.4
作者：
Dumoulin, A;Lévi, S;Triller, A
通讯作者：
Triller, A