Plasma-activated water: An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection.
Plasma-activated water: An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection.
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DOI：
10.1016/j.cej.2020.127742
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发表时间：
2021-10-01
期刊：
影响因子：
--
通讯作者：
Kong MG
中科院分区：
文献类型：
--
作者：
Guo L;Yao Z;Yang L;Zhang H;Qi Y;Gou L;Xi W;Liu D;Zhang L;Cheng Y;Wang X;Rong M;Chen H;Kong MG
SARS-CoV-2是一种高度传染性的病毒，正在引发一场全球大流行。SARS-CoV-2的感染依赖于细胞受体人血管紧张素转换酶2(HACE2)的识别和结合，阻断这一过程可以有效地抑制SARS-CoV-2的侵袭。等离子体活化水通过破坏生物大分子有效地灭活细菌和噬菌体，但其对冠状病毒的作用尚未见报道。本研究以含有SARS-CoV-2 S蛋白的假病毒为模型，用血浆活化水通过灭活S蛋白有效地抑制了假病毒的感染。用RBD研究了RBD的分子细节，并用等离子体活化水通过RBD修饰使RBD结合活性失活。PAW中的短命活性物种，如ONOO−，在这种失活中起着至关重要的作用。室温保存30天后，血浆活化水仍能显著降低RBD与hACE2的结合。总之，我们的发现为抗击SARS-CoV-2引起的流行病的有效消毒策略提供了证据。
SARS-CoV-2 is a highly contagious virus and is causing a global pandemic. SARS-CoV-2 infection depends on the recognition of and binding to the cellular receptor human angiotensin-converting enzyme 2 (hACE2) through the receptor-binding domain (RBD) of the spike protein, and disruption of this process can effectively inhibit SARS-CoV-2 invasion. Plasma-activated water efficiently inactivates bacteria and bacteriophages by causing damage to biological macromolecules, but its effect on coronavirus has not been reported. In this study, pseudoviruses with the SARS-CoV-2 S protein were used as a model, and plasma-activated water (PAW) effectively inhibited pseudovirus infection through S protein inactivation. The RBD was used to study the molecular details, and the RBD binding activity was inactivated by plasma-activated water through the RBD modification. The short-lived reactive species in the PAW, such as ONOO−, played crucial roles in this inactivation. Plasma-activated water after room-temperature storage of 30 days remained capable of significantly reducing the RBD binding with hACE2. Together, our findings provide evidence of a potent disinfection strategy to combat the epidemic caused by SARS-CoV-2.
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发表时间：
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