Decolonisation of MRSA, S. aureus and E. coli by cold-atmospheric plasma using a porcine skin model in vitro.
Decolonisation of MRSA, S. aureus and E. coli by cold-atmospheric plasma using a porcine skin model in vitro.
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DOI：
10.1371/journal.pone.0034610
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zimmermann JL
中科院分区：
文献类型：
--
作者：
Maisch T;Shimizu T;Li YF;Heinlin J;Karrer S;Morfill G;Zimmermann JL
近二十年来，美国FDA批准的新型抗菌药呈下降趋势，而多重耐药菌的耐药性却呈上升趋势。因此，社区和医院获得性耐药细菌感染导致标准治疗的疗效下降，延长治疗时间，增加医疗费用。因此，这项工作的目的是证明冷大气等离子体在体外猪皮模型中对革兰氏阳性菌(耐甲氧西林金黄色葡萄球菌(MRSA)和甲氧西林敏感金黄色葡萄球菌(MRSA))和革兰氏阴性菌(大肠杆菌)的去克隆的适用性。新鲜切除的皮肤样本取自6个月大的母猪(品种：皮特兰)。在外植体表面涂抹纯细菌后，用冷大气等离子体处理长达15分钟。对两种不同的等离子体装置进行了评估。在6分钟的等离子体处理后，已经达到了3log10步的去克隆效果。较长的等离子体处理时间可获得5log10步的杀灭率，而不依赖于所应用的细菌菌株。低温常压等离子体处理24 h内未处理和处理皮肤的组织学评价表明，未处理和处理的皮肤没有形态变化，也没有明显程度的坏死或细胞凋亡，表明猪皮肤仍然是有生命的。这项研究首次证明，冷大气等离子体能够非常有效地杀灭施加在完整皮肤表面的细菌，使用的是体外猪皮肤模型。这些结果强调了冷大气等离子体作为一种新的可能的治疗选择的潜力，用于未来在不损害周围组织的情况下将人类皮肤从患者的细菌中分离出来。
In the last twenty years new antibacterial agents approved by the U.S. FDA decreased whereas in parallel the resistance situation of multi-resistant bacteria increased. Thus, community and nosocomial acquired infections of resistant bacteria led to a decrease in the efficacy of standard therapy, prolonging treatment time and increasing healthcare costs. Therefore, the aim of this work was to demonstrate the applicability of cold atmospheric plasma for decolonisation of Gram-positive (Methicillin-resistant Staphylococcus aureus (MRSA), Methicillin-sensitive Staphylococcus aureus) and Gram-negative bacteria (E. coli) using an ex vivo pig skin model. Freshly excised skin samples were taken from six month old female pigs (breed: Pietrain). After application of pure bacteria on the surface of the explants these were treated with cold atmospheric plasma for up to 15 min. Two different plasma devices were evaluated. A decolonisation efficacy of 3 log10 steps was achieved already after 6 min of plasma treatment. Longer plasma treatment times achieved a killing rate of 5 log10 steps independently from the applied bacteria strains. Histological evaluations of untreated and treated skin areas upon cold atmospheric plasma treatment within 24 h showed no morphological changes as well as no significant degree of necrosis or apoptosis determined by the TUNEL-assay indicating that the porcine skin is still vital. This study demonstrates for the first time that cold atmospheric plasma is able to very efficiently kill bacteria applied to an intact skin surface using an ex vivo porcine skin model. The results emphasize the potential of cold atmospheric plasma as a new possible treatment option for decolonisation of human skin from bacteria in patients in the future without harming the surrounding tissue.
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