Altered prostate epithelial development and IGF-1 signal in mice lacking the androgen receptor in stromal smooth muscle cells.
Altered prostate epithelial development and IGF-1 signal in mice lacking the androgen receptor in stromal smooth muscle cells.
复制标题
基质平滑肌细胞中缺乏雄激素受体的小鼠的前列腺上皮发育和 IGF-1 信号发生改变。
DOI：
10.1002/pros.21264
复制
发表时间：
2011-04
期刊：
影响因子：
2.8
通讯作者：
Yeh, Shuyuan
中科院分区：
文献类型：
--
作者：
Yu, Shengqiang;Zhang, Caixia;Lin, Chiu-Chun;Niu, Yuanjie;Lai, Kuo-Pao;Chang, Hong-chiang;Yeh, Shauh-Der;Chang, Chawnshang;Yeh, Shuyuan
Androgens and the androgen receptor (AR) play critical roles in the prostate development via mesenchymal-epithelial interactions. Smooth muscle cells, differentiated from mesenchyme, are one of the basic components of the prostate stroma. However, the roles of smooth muscle AR in prostate development are still obscure. We established the smooth muscle selective AR knockout (SM-ARKO) mouse model using the Cre-loxP system, and confirmed the AR knockout efficiency at RNA, DNA and protein levels. Then we observed the prostate morphology changes, and determined the epithelial proliferation, apoptosis, and differentiation. We also knocked down the AR in a prostate smooth muscle cell line (PS-1) to confirm the in vivo findings and to probe the mechanism. The AR was selectively and efficiently knocked out in the anterior prostates of SM-ARKO mouse. The SM-ARKO prostates have defects with loss of infolding structures, and decrease of epithelial proliferation, but with little change of apoptosis and differentiation. The mechanism studies showed that IGF-1 expression level decreased in the SM-ARKO prostates and AR-knockdown PS-1 cells. The decreased IGF-1 expression might contribute to the defective development of SM-ARKO prostates. The AR in smooth muscle cells plays important roles in the prostate development via the regulation of IGF-1 signal.
登录
查看更多内容
影响因子：
6.6
作者：
SHAPIRO, E;BECICH, MJ;LEPOR, H
通讯作者：
LEPOR, H
DOI：
10.1073/pnas.0608556103
发表时间：
2006-11-21
影响因子：
11.1
作者：
Zhang, Caixia;Yeh, Shuyuan;Chang, Chawnshang
通讯作者：
Chang, Chawnshang
影响因子：
4.8
作者：
COOKE, PS;YOUNG, P;CUNHA, GR
通讯作者：
CUNHA, GR
影响因子：
5
作者：
Frutkin, Andrew D.;Shi, Haikun;Dichek, David A.
通讯作者：
Dichek, David A.
DOI：
10.1073/pnas.0704940104
发表时间：
2007-07-31
影响因子：
11.1
作者：
Wu, Chun-Te;Altuwaijri, Saleh;Chang, Chawnshang
通讯作者：
Chang, Chawnshang