Cytokeratin in early hamster embryogenesis and parthenogenesis: reorganization during mitosis and association with clusters of interchromatinlike granules.

Cytokeratin in early hamster embryogenesis and parthenogenesis: reorganization during mitosis and association with clusters of interchromatinlike granules.
复制标题

早期仓鼠胚胎发生和孤雌生殖中的细胞角蛋白:有丝分裂期间的重组以及与染色质间样颗粒簇的关联。

DOI:
--
复制
发表时间:
1991
期刊:
Differentiation; research in biological diversity
影响因子:
--
通讯作者:
J. David
J. David
中科院分区:
--
文献类型:
--
作者:
C. Plancha;M. Carmo;J. David

文献摘要

参考文献

被引文献

相似文献

利用体外获得的金仓鼠胚胎,通过免疫荧光和免疫电镜研究了细胞角蛋白在完成减数分裂和第一次卵裂分裂过程中的重排。我们的研究结果指出了三个主要的重组步骤:(1)在单细胞胚胎中,最近排卵的卵母细胞特征的弥漫性免疫荧光细胞角蛋白斑点重新排列成由原纤维相互连接的大皮质斑块;(2)有丝分裂时,出现均匀的细胞角蛋白斑点;(3)在双细胞胚胎中,皮层和核周细胞角蛋白纤维网络变得突出。孤雌生殖卵母细胞能够模仿在第一次胚胎有丝分裂之前观察到的主要细胞角蛋白模式,这支持了母体建立的对激活的共同反应的概念。尽管在有丝分裂过程中缺乏纤维免疫荧光反应性,但电子显微镜显示10纳米丝网的持久性。这些细胞角蛋白网通常与染色质间样颗粒簇有关,这些颗粒在核膜重组后在细胞质中持续存在一段时间。
In vivo obtained golden hamster embryos were used to study, by immunofluorescence and immunoelectron microscopy, the main cytokeratin pattern rearrangements during completion of meiosis and the first cleavage division. Our results point to three major re-organization steps: (1) diffuse immunofluorescent cytokeratin spots characteristic of recently ovulated oocytes rearrange into large cortical patches interconnected by fibrils in one-cell embryos; (2) during mitosis a homogeneous cytokeratin spotty pattern reappears; (3) in two-cell embryos cortical and perinuclear cytokeratin fibrillar networks become prominent. Parthenogenotic oocytes were able to mimic the major cytokeratin patterns observed until the first embryonic mitosis, supporting the concept of a maternally established common response to activation. Despite the lack of fibrillar immunofluorescent reactivity during mitosis, electron microscopy demonstrates persistence of 10 nm filament meshworks. These cytokeratin meshworks often associate with clusters of interchromatinlike granules, which persist in the cytoplasm for a short period after nuclear envelope reassembly.
伴随小鼠卵母细胞减数分裂成熟和小鼠卵子受精的蛋白质磷酸化的阶段特异性变化。
DOI: 10.1002/jez.1402390311
发表时间: 1986
期刊: The Journal of experimental zoology
影响因子: --
作者:
Endo,Y;Kopf,GS;Schultz,RM
通讯作者: Schultz,RM
DOI: --
发表时间: 1989
影响因子: 6.6
作者:
Leser,GP;Fakan,S;Martin,TE
通讯作者: Martin,TE
DOI: 10.1016/0922-3371(90)90128-j
发表时间: 1990
期刊: Cell differentiation and development : the official journal of the International Society of Developmental Biologists
影响因子: --
作者:
Seshagiri,PB;Bavister,BD;Williamson,JL;Aiken,JM
通讯作者: Aiken,JM