Expression of noradrenergic and cholinergic traits by sympathetic neurons cultured without serum
Expression of noradrenergic and cholinergic traits by sympathetic neurons cultured without serum
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无血清培养的交感神经元去甲肾上腺素能和胆碱能特征的表达
DOI：
10.1523/jneurosci.05-06-01497.1985
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发表时间：
1985
影响因子：
2.7
通讯作者：
P. Patterson
中科院分区：
文献类型：
--
作者：
E. Wolinsky;S. Landis;P. Patterson
系统地改变神经元环境的能力是使用细胞培养提供的一个优点。用已知成分替代血清（一种可变和不确定的培养基补充剂）可以更好地控制培养条件。我们研究了在改良的限定培养基中培养的大鼠交感神经元与神经递质代谢相关的生化和形态学特性。神经元的存活，超微结构和去甲肾上腺素能特性的表达似乎在无血清和血清补充的文化：小颗粒囊泡特征的去甲肾上腺素存储中观察到两种类型的文化，酪氨酸羟化酶活性，多巴胺转化为去甲肾上腺素，儿茶酚胺生产，和存储能力是等同的无血清和含血清的文化。这些特性中的一些在该成分确定的培养基的先前配方中没有以高水平显示。然而，与补充有血清的培养物相比，无血清培养物中的乙酰胆碱产量低约10倍，这与lacovitti等人的发现一致（lacovitti，L.，M. I.约翰逊，T. H. Joh和R. P. Bunge（1982）Neuroscience 7：2225-2239）。在无血清条件下，通过先前表征的来自心脏细胞条件培养基的胆碱能诱导因子可以诱导乙酰胆碱产生。这种无血清心脏细胞条件培养基的反应表明，无血清文化保留可塑性相对于发射机的状态，尽管表达的去甲肾上腺素能的特性，不像培养的神经元的去甲肾上腺素能递质的状态是由慢性去极化。因此，交感神经元在无血清条件下存活，表达许多分化的特性，并显示新的递质状态。
The ability to vary systematically the neuronal environment is one advantage afforded by the use of cell culture. Replacement of serum, a variable and undefined medium supplement, with known ingredients allows even greater control of culture conditions. We have studied biochemical and morphological properties related to neurotransmitter metabolism of rat sympathetic neurons cultured in a modified defined medium. Neuronal survival, ultrastructure, and expression of noradrenergic properties appear similar in serum-free and serum-supplemented cultures: small granular vesicles characteristic of norepinephrine storage were observed in both types of culture, and tyrosine hydroxylase activity, conversion of dopamine to norepinephrine, catecholamine production, and storage capacity are equivalent in serum-free and serum-containing cultures. Several of these properties were not exhibited at high levels in previous formulations of this defined medium. Acetylcholine production, however, was about 10-fold lower in serum-free compared to serum-supplemented cultures, consistent with the findings of lacovitti et al. (lacovitti, L., M. I. Johnson, T. H. Joh, and R. P. Bunge (1982) Neuroscience 7:2225–2239). Acetylcholine production can be induced under serum-free conditions by a previously characterized cholinergic inducing factor from heart cell conditioned medium. This responsiveness to serum-free heart cell conditioned medium indicates that serum-free cultures retain plasticity with respect to transmitter status, despite expression of noradrenergic characteristics, unlike cultured neurons of which the noradrenergic transmitter status is maintained by chronic depolarization. Thus, sympathetic neurons survive, express numerous differentiated properties, and display a novel transmitter status under serum-free conditions.
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S49 淋巴瘤细胞中血清诱导的环 AMP 通路改变的特征。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Darfler,FJ;Hughes,RJ;Insel,PA
通讯作者：
Insel,PA
体内神经递质可塑性的证据：胆碱能交感神经元特性的发育变化。
影响因子：
2.7
作者：
Landis,SC;Keefe,D
通讯作者：
Keefe,D
无血清培养的小鸡交感神经元和感觉神经元长期存活所需的底物要求和培养基补充。
影响因子：
3.7
作者：
Wakade,AR;Edgar,D;Thoenen,H
通讯作者：
Thoenen,H
负责测定培养的交感神经元中胆碱能功能的扩散因子。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Weber,MJ
通讯作者：
Weber,MJ