NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement across Capillaries and into Cardiomyocytes.
NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement across Capillaries and into Cardiomyocytes.
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DOI：
10.1016/j.cmet.2018.03.017
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发表时间：
2018-05-01
期刊：
影响因子：
29
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
He C;Weston TA;Jung RS;Heizer P;Larsson M;Hu X;Allan CM;Tontonoz P;Reue K;Beigneux AP;Ploug M;Holme A;Kilburn M;Guagliardo P;Ford DA;Fong LG;Young SG;Jiang H
富含甘油三酯的脂蛋白(TRL)在毛细血管中的加工为重要组织提供脂质，但我们对TRL代谢的了解有限，部分原因是TRL的加工和脂质运动从未被可视化。为了研究TRL衍生的脂类在心脏中的运动，给小鼠注射富含[2H]甘油三酯的TRL，并用NanoSIMS检测2H标记的脂类跨毛细血管和进入心肌细胞的运动。Trl在组织中的加工和脂质运动非常迅速。在30秒内，TRL衍生的脂质出现在心肌细胞的内皮下间隙、脂滴和线粒体中。2H在毛细血管内皮细胞中的浓集程度不高于心肌细胞，提示内皮细胞可能不是脂质进入心肌细胞的控制点。值得注意的是，在毛细血管内皮细胞中高表达的假定脂肪酸转运蛋白CD36的缺失并没有阻碍TRL衍生的脂类进入心肌细胞。XXX等人使用NanoSIMS可视化了富含甘油三酯的脂蛋白(TRL)衍生的脂类在心脏中的运动。Trl衍生的脂类、线粒体和脂滴在几秒钟内。此外，CD36的缺失不会阻碍TRL衍生的脂类进入心肌细胞。
The processing of triglyceride-rich lipoproteins (TRLs) in capillaries provides lipids for vital tissues, but our understanding of TRL metabolism is limited, in part because TRL processing and lipid movement have never been visualized. To investigate the movement of TRL-derived lipids in the heart, mice were given an injection of [2H]triglyceride-enriched TRLs, and the movement of 2H-labeled lipids across capillaries and into cardiomyocytes was examined by NanoSIMS. TRL processing and lipid movement in tissues was extremely rapid. Within 30 sec, TRL-derived lipids appeared in the subendothelial spaces and in the lipid droplets and mitochondria of cardiomyocytes. 2H enrichment in capillary endothelial cells was not greater than in cardiomyocytes, implying that endothelial cells may not be a control point for lipid movement into cardiomyocytes. Remarkably, a deficiency of the putative fatty acid transport protein CD36, which is expressed highly in capillary endothelial cells, did not impede entry of TRL-derived lipids into cardiomyocytes. XXX et al used NanoSIMS to visualize the movement of triglyceride-rich lipoproteins (TRLs)-derived lipids in the heart. TRL-derived lipids mitochondria and lipid droplets within seconds. Also, loss of CD36 did not impede entry of TRL-derived lipids into cardiomyocytes.
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影响因子：
29
作者：
Goulbourne CN;Gin P;Tatar A;Nobumori C;Hoenger A;Jiang H;Grovenor CR;Adeyo O;Esko JD;Goldberg IJ;Reue K;Tontonoz P;Bensadoun A;Beigneux AP;Young SG;Fong LG
通讯作者：
Fong LG
影响因子：
64.8
作者：
Hagberg, Carolina E.;Falkevall, Annelie;Eriksson, Ulf
通讯作者：
Eriksson, Ulf
DOI：
10.1007/bf01033631
发表时间：
1982-01-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
ANGERMULLER, S;FAHIMI, HD
通讯作者：
FAHIMI, HD
DOI：
10.1152/ajpgi.00386.2005
发表时间：
2006-03-01
影响因子：
4.5
作者：
Guo, W;Huang, N;Hamilton, JA
通讯作者：
Hamilton, JA
影响因子：
6.5
作者：
Jiang, Haibo;Goulbourne, Chris N.;Young, Stephen G.
通讯作者：
Young, Stephen G.