Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota.
Lantibiotics Produced by Oral Inhabitants as a Trigger for Dysbiosis of Human Intestinal Microbiota.
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DOI：
10.3390/ijms22073343
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发表时间：
2021-03-25
影响因子：
5.6
通讯作者：
Kamiya S
中科院分区：
文献类型：
--
作者：
Yonezawa H;Motegi M;Oishi A;Hojo F;Higashi S;Nozaki E;Oka K;Takahashi M;Osaki T;Kamiya S
羊毛硫抗生素是由革兰氏阳性菌产生的一类细菌素，具有广谱的革兰氏阳性抗菌活性。在这项研究中，我们确定了主要由广泛的致龋细菌变形链球菌产生的Mutacin I/III和Smb（一种二肽lantibiotic）对构成肠道微生物群的许多革兰氏阳性菌具有很强的抗菌活性。这些羊毛硫抗生素还表现出对酸和温度的抗性。基于这些特征，我们预测羊毛硫抗生素可能能够持续进入肠道，保持较强的抗微生物活性，影响肠道微生物群。收集了69名受试者的唾液和粪便样本以检验这一假设，并检查了羊毛硫抗生素的存在和肠道微生物群的组成。我们证明，在他们的口腔中拥有产乳酸菌的受试者表现出物种丰富度下降的趋势，并在他们的肠道微生物群中显着减少了厚壁菌门的丰度。在喂食S.含有lantibiotic细菌素Mutacin I的变形菌培养物上清液。这些结果表明，在口腔中产生的lantibiotic细菌素扰乱肠道微生物群，并表明口腔细菌可能是肠道微生物群失调的致病因素之一。
Lantibiotics are a type of bacteriocin produced by Gram-positive bacteria and have a wide spectrum of Gram-positive antimicrobial activity. In this study, we determined that Mutacin I/III and Smb (a dipeptide lantibiotic), which are mainly produced by the widespread cariogenic bacterium Streptococcus mutans, have strong antimicrobial activities against many of the Gram-positive bacteria which constitute the intestinal microbiota. These lantibiotics also demonstrate resistance to acid and temperature. Based on these features, we predicted that lantibiotics may be able to persist into the intestinal tract maintaining a strong antimicrobial activity, affecting the intestinal microbiota. Saliva and fecal samples from 69 subjects were collected to test this hypothesis and the presence of lantibiotics and the composition of the intestinal microbiota were examined. We demonstrate that subjects possessing lantibiotic-producing bacteria in their oral cavity exhibited a tendency of decreased species richness and have significantly reduced abundance of the phylum Firmicutes in their intestinal microbiota. Similar results were obtained in the fecal microbiota of mice fed with S. mutans culture supernatant containing the lantibiotic bacteriocin Mutacin I. These results showed that lantibiotic bacteriocins produced in the oral cavity perturb the intestinal microbiota and suggest that oral bacteria may be one of the causative factors of intestinal microbiota dysbiosis.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
3.7
作者：
Merritt J;Qi F
通讯作者：
Qi F
影响因子：
4.4
作者：
Escano, Jerome;Ravichandran, Akshaya;Smith, Leif
通讯作者：
Smith, Leif
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
5.4
作者：
Chihib, NE;Tholozan, JL
通讯作者：
Tholozan, JL