1,25-Dihydroxyvitamin D3 modulates colony-stimulating factor-1 receptor binding by murine bone marrow macrophage precursors.
1,25-Dihydroxyvitamin D3 modulates colony-stimulating factor-1 receptor binding by murine bone marrow macrophage precursors.
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1,25-二羟基维生素 D3 通过小鼠骨髓巨噬细胞前体调节集落刺激因子 1 受体结合。
DOI：
10.1210/endo-128-1-303
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发表时间：
1991
期刊：
影响因子：
4.8
通讯作者：
Steven L. Teitelbaum
中科院分区：
文献类型：
--
作者：
Sherrie L. Perkins;Steven L. Teitelbaum
1，25-二羟基维生素D3 [1，25-（OH）2D 3]已被证明是白血病细胞系中单核细胞/巨噬细胞分化的有效试剂。本研究检测了1，25-（OH）2D 3对5-FU预处理小鼠骨髓收集的真实小鼠骨髓未成熟未定型前体细胞的分化作用。5-FU处理后1天收集的早期前体细胞的特征在于集落刺激因子-1的低表达。（CSF-I）受体和对CSF-I和白介素-1两者的生长依赖性。在5-FU处理后5天收集中间前体，其生长需要CSF-1或白细胞介素-3。中间前体表达相对较高水平的CSF-1受体。此外，1，25-（OH）2D 3这些人口抑制增殖，测量[3 H]胸苷掺入和软琼脂集落测定。此外，1，25-（OH）2D 3以剂量依赖性代谢物特异性方式导致早期前体细胞中CSF-1受体更快出现。相反，CSF-1受体的表达减少，在中间前体治疗的类固醇。这种受体表达的降低也具有剂量依赖性和代谢物特异性。这些发现表明，1，25-（OH）2D 3 1）靶向不同分化阶段的真正骨髓巨噬细胞，2）调节CSF-1受体的表达，CSF-1受体是一种反过来调节单核细胞成熟的蛋白质。
1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] has been shown to be a potent agent for monocyte/macrophage differentiation in leukemic cell lines. This study examines the differentiational effects of 1,25-(OH)2D3 in authentic murine bone marrow immature uncommitted precursor cells collected by pretreatment of murine bone marrow with 5-fluorouracil (5-FU). Early precursor cells, collected 1 day after 5-FU treatment, are characterized by low expression of the colony-stimulating factor-1. (CSF-1) receptor and dependence on both CSF-1 and interleukin-1 for growth. Intermediate precursors were collected 5 days after 5-FU treatment and required either CSF-1 or interleukin-3 for growth. Intermediate precursors expressed relatively higher levels of the CSF-1 receptor. Addition of 1,25-(OH)2D3 to these populations inhibited proliferation, as measured by [3H]thymidine incorporation and soft agar colony assay. Furthermore, 1,25-(OH)2D3 caused a more rapid appearance of the CSF-1 receptor in early precursor cells in a dose-dependent metabolite-specific manner. Conversely, CSF-1 receptor expression was decreased in intermediate precursors treated with the steroid. This decrease in receptor expression was also dose dependent and metabolite specific. These findings demonstrate that 1,25-(OH)2D3 1) targets to authentic bone marrow macrophages at various stages of differentiation and 2) modulates expression of the CSF-1 receptor, a protein which, in turn, regulates monocytic maturation.
影响因子：
20.3
作者：
K. Koike;E. Stanley;J. Ihle;M. Ogawa
通讯作者：
K. Koike;E. Stanley;J. Ihle;M. Ogawa
人单核细胞分化过程中巨噬细胞特异性集落刺激因子 (CSF-1) 的表达。
DOI：
10.1016/s0006-291x(86)80131-0
发表时间：
1986
影响因子：
3.1
作者：
Horiguchi,J;Warren,MK;Ralph,P;Kufe,D
通讯作者：
Kufe,D