Amplification of Signaling Activity of the Arc Two-component System of Escherichia coli by Anaerobic Metabolites
Amplification of Signaling Activity of the Arc Two-component System of Escherichia coli by Anaerobic Metabolites
复制标题
厌氧代谢物放大大肠杆菌弧双组分系统的信号活性
DOI：
--
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发表时间：
1999
影响因子：
4.8
通讯作者：
E. Lin
中科院分区：
文献类型：
--
作者：
D. Georgellis;O. Kwon;E. Lin
在大肠杆菌中，Arc 双组分系统可感知并发出生长氧化还原条件的变化。该系统由作为膜相关传感器激酶的 ArcB 和作为细胞质反应调节器的 ArcA 组成。 ArcB 是一种三联激酶，具有初级递质、受体和次级递质域，可通过 His → Asp → His → Asp 磷酸中继催化 ArcA 的磷酸化，以及通过反向磷酸中继催化 ArcA-P 的去磷酸化。当 ArcA 和 ArcB 与 ATP 一起孵育时，磷酸化蛋白的峰值水平在发酵代谢物（乳酸、乙酸或丙酮酸）的存在下增加。在本研究中，我们报告这些效应子加速了 ArcB 的自磷酸化活性并增强了 ArcA 的转磷酸化，但对 ArcA-P 的去磷酸化没有影响。此外，ArcB 接收域的存在对于效应器影响 ArcB 主要发射域的自磷酸化速率至关重要。
In Escherichia coli, changes in redox condition of growth are sensed and signaled by the Arc two-component system. This system consists of ArcB as the membrane-associated sensor kinase and ArcA as the cytoplasmic response regulator. ArcB is a tripartite kinase, possessing a primary transmitter, a receiver, and a secondary transmitter domain that catalyzes the phosphorylation of ArcA via a His → Asp → His → Asp phosphorelay, as well as the dephosphorylation of ArcA-P by a reverse phosphorelay. When ArcA and ArcB were incubated with ATP, the peak levels of phosphorylated proteins increased in the presence of the fermentation metabolitesd-lactate, acetate, or pyruvate. In this study, we report that these effectors accelerate the autophosphorylation activity of ArcB and enhance the transphosphorylation of ArcA, but have no effect on the dephosphorylation of ArcA-P. Moreover, the presence of the receiver domain of ArcB is essential for the effectors to influence the autophosphorylation rate of the primary transmitter domain of ArcB.
DOI：
10.1073/pnas.85.6.1888
发表时间：
1988-03-01
影响因子：
11.1
作者：
IUCHI, S;LIN, ECC
通讯作者：
LIN, ECC
接收器模块在保守天冬氨酸残基处的磷酸化/去磷酸化控制大肠杆菌传感器蛋白 ArcB 中组氨酸激酶的转磷酸化活性。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Iuchi,S
通讯作者：
Iuchi,S